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October 1955

Histologic Study of Fungus Cultures

Author Affiliations

New York

From the Department of Medicine (Dermatology), New York Hospital and Cornell University Medical Center.

AMA Arch Derm. 1955;72(4):362-370. doi:10.1001/archderm.1955.03730340060011

In the identification of the dermatophytes reliance is placed chiefly on the gross characteristics of the culture. These features may be sufficiently distinctive so that additional procedures are unnecessary (i. e., Microsporum lanosum, Epidermophyton floccosum, and others). However, this is not true with some other fungi, and further investigation of the culture is then desirable. This is usually carried out by means of microscopic examination of a microculture or of material removed from the culture. The present study was undertaken to explore the possibility that histologic preparations of fungus colonies might help in their recognition.


Source of Specimen.—Twelve common dermatophytes were chosen for study. At least three different strains of each species were used; most had been recently isolated. Six common contaminants were examined.

Culture Medium.—The fungi were grown at room temperature in 4 in. (10 cm.) Petri dishes, the medium consisting