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Article
December 1956

A NEW CULTURE MEDIUM FOR CANDIDIASIS

AMA Arch Derm. 1956;74(6):678. doi:10.1001/archderm.1956.01550120098023
Abstract

The purpose of this report is to describe a new and effective culture medium for the detection of Candida in dermatology. In patients with axillary and groin dermatitides and with paronychial involvement of the nail, candidiasis may be a primary or a secondary factor. A rapid culture would be of invaluable aid as to the choice of therapy.

The active ingredient of Nickersons Medium* is a complex polybismuth sulfite. On this medium, Candida albicans grows in large, jet-black or dark brown, shiny, hemispherical colonies, with peripheral filamentation upon continued incubation. Other species of the genus Candida grow very dark brown in color. This medium will not support bacterial growth. After inoculation, either with sterile swabs or the usual inoculation technique, Candida cultures appear at room temperature within five days. The vials are small, only 2 in. long, and do not constitute a space problem in the office.

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