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April 1960

The Cultivation of Dermatophytes on the Air Cell Membrane of the Hen's Egg

Author Affiliations

Leiden, Netherlands

From the Mycological Laboratory (Head: R. H. Cormane, M.D.) of the Department of Dermatology (Director: Prof. Dr. H. W. Siemens), University Hospital, Leiden.

AMA Arch Derm. 1960;81(4):506-508. doi:10.1001/archderm.1960.03730040010002

Introduction  In 1957, I. Neuhauser reported in this journal that the air cell membrane of the hen's egg is especially suitable as a medium for the cultivation of dermatophytes. This keratinous membrane is moist, is protected by the shell of the egg, and need not be sterilized. According to Neuhauser it shows a physical and chemical similarity to the human epidermis, notably because of its high methionine content, which renders this medium exceedingly suitable for the study of dermatophytes. In the author's view the membrane has a certain specificity as a culture medium for dermatophytes, and an inhibitory action on the development of bacteria present in the material to be mycologically examined.This culture medium is low in price, and, being also easily obtainable as well as readily rendered suitable for the cultivation of fungi, it might be most serviceable in the mycological investigation of hair, nails, epithelial scales, etc.

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