Acridine orange has been used for some time as a fluorescent stain for nucleoproteins and for tissue mucopolysaccharides.6 It has also found use as a vital stain for blood parasites.8By accident it was found that the technique of Hicks and Matthaei for the fluorescent staining of mucin4 would also stain fungi in tissue sections which would then fluoresce under blue-violet light.7 This finding led to a study of further applications of this method to demonstrate structural details of the fungi themselves and as diagnostic aids in clinical evaluations.
Materials and Methods
Microscope and Filters.—
These studies were done using a Reichert Zetopan microscope with fluorescent attachments. Results were checked periodically with an ordinary binocular microscope and lamp equipped with filters for fluorescent microscopy7 to insure adaptability to this apparatus. In the latter apparatus a Leitz or Zeiss bluelight fluorescence filter (BG 12, 4 mm.
CHICK EW. Acridine Orange Fluorescent Stain for Fungi. Arch Dermatol. 1961;83(2):305–309. doi:10.1001/archderm.1961.01580080135015
Customize your JAMA Network experience by selecting one or more topics from the list below.
Create a personal account or sign in to: