The exact site of the antigen-antibody reaction in allergic contact dermatitis is unknown. Early investigators were proponents of the humoral theory, believing that the site of the antigen-antibody reaction is in the blood stream. Supporters of the currently more popular cellular theory offer evidence to indicate that the site is in or on the tissue cells.16 In 1942, Coons and co-workers first demonstrated the value of using a fluorescent antibody to identify antigens in tissue.1 In the past decade there have been many refinements in the procedure,3 and the technique has been used successfully in the identification of micro-organisms5,6,10,11 and in the immunohistochemical investigation of connective tissue diseases and many other pathologic states.14,17 This paper reports the successful results of application of the technique to localize the site of the antigenantibody reaction in human subjects in experimentally produced Rhus dermatitis.
Materials and Methods
RASKIN J. Antigen-Antibody Reaction Site in Contact Dermatitis: Determination by Use of Fluorescent Antibody Technique. Arch Dermatol. 1961;83(3):459–465. doi:10.1001/archderm.1961.01580090109014
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