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July 1961

Fluorescent Antibody Studies in Chronic Dermatitis

Author Affiliations


Department of Medicine, Division of Dermatology and Division of Gastroenterology, Tulane University School of Medicine and Charity Hospital, senior student, Tulane University School of Medicine (Mr. Shames).

Arch Dermatol. 1961;84(1):37-39. doi:10.1001/archderm.1961.01580130043006

The fluorescent antibody technique originated by Dr. Albert H. Coons1 provides an excellent method for localization of antigen-antibody reactions in tissue sections. Globulin molecules are conjugated with a dye which renders them fluorescent when viewed through a special microscope. The antibody, thus labeled, is employed as a histochemical stain for the demonstration of antigen.2

The method is applicable either as an indirect method utilizing tagged antiglobulin antiserum to demonstrate prefixed antibody or as a direct method in which specific antiserum is tagged and reacted directly with the antigen. With either method, one is obviously dealing with a spectrum of antigen-antibody systems unless specific absorptions are performed, and the results must be interpreted accordingly.

In the following study the direct method of Coons was employed in selected cases of chronic dermatitis. Fourteen patients were tested using labeled antiserum and autologous skin sections.

Method  A detailed account of the procedure

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