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August 1961

Appraisal of Sabouraud's, Cycloheximide, and Oxgall Agars

Author Affiliations


From the Division of Dermatology, Department of Medicine, State University of New York, Downstate Medical Center, Brooklyn.

Arch Dermatol. 1961;84(2):256-260. doi:10.1001/archderm.1961.01580140082010

The need to improve the isolation technique for pathogenic fungi has prompted the use of nutrient media designed selectively to inhibit nonpathogenic micro-organisms without interfering with the growth of pathogenic fungi. While many of the selective media recommended hardly passed the stage of initial introduction, 2 received increasing attention and acclaim, namely, Littman's1 oxgall medium and Georg's2 cycloheximide agar. Their respective merits were appraised in a number of publications. A few are cited in the following: Littman, McQuown, and Schneidau,3 in 1949, stated that in 222 cases of systemic and cutaneous mycoses 5 to 6 times as many isolates were discovered on oxgall medium as on Sabouraud's dextrose agar. Georg,2 in 1953, reported that in cultures from 30 persons with suspected onychomycosis 3 times as many isolates were obtained on cycloheximide medium as on Sabouraud's agar. Shapiro, Mullins, and Pinkerton,4 in 1956, performing duplicate cultures