The immediate precursor of homogentisic acid has been the subject of speculation since Neubauer first postulated the metabolic pathways from phenylalanine and tyrosine to the citric acid cycle.1-10 In fact not all features of this theoretical flowsheet have as yet been substantiated by experimental evidence. The occurrence of 2,5 dihydroxyphenylpyruvic acid (2,5 DHPPA) in human urine has remained largely hypothetical. In 1958, Nishimura and coworkers11-12 demonstrated for the first time the presence of 2,5 DHPPA in the urine of patients with connective tissue diseases. However, the method used by them for detecting the 2,5 DHPPA was qualitative and subject to question with respect to the amount and identity of the material found. In 1959 Tye et al.,13 using ultraviolet spectrophotometry and paper chromatography, reported that 2,5 DHPPA was not detected by their methods. This paper is concerned with presenting evidence which supports the view that 2,5 DHPPA
YEE HY, McCANN DS, KEECH MK, DENKO CW, BOYLE AJ. 2,5 Dihydroxyphenylpyruvic Acid in Human Urine: Preliminary Identification. Arch Dermatol. 1961;84(2):293–301. doi:10.1001/archderm.1961.01580140119015
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