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June 1962

Aminopeptidase in Elastotic Skin

Author Affiliations


From the Northwestern University Medical Center, Department of Dermatology, and the Department of Medicine (Dermatology) of the Veterans Administration Research Hospital.

Arch Dermatol. 1962;85(6):720-721. doi:10.1001/archderm.1962.01590060030005

Although peptidases have been found to be localized principally in the cellular, as opposed to the fibrous, elements of the skin,1,2 the presence of an aminopeptidase in the fibrous dermal structure in elastotic skin has not been reported. An aminopeptidase can indeed be demonstrated in the elastotic skin by histochemical staining methods which utilize L-leucyl-β-naphthylamide as the substrate. This study was done on the cutaneous biopsy specimens from 10 patients as part of an investigation of the exopeptidases of the skin.

Material and Method  Cutaneous punch biopsy specimens from the face and neck of 10 white male patients with elastosis of the skin, whose ages ranged from 45 to 72, were frozen in isopentane in liquid nitrogen and sectioned at 16μ thickness in a cryostat. The sections were stained for aminopeptidase by the modification of Nachlas, Crawford, and Seligman. Control slides on each specimen were placed (a) in substrate