Swartz and Lamkins combined dioctyl sodium sulfosuccinate (an ionic wetting agent), potassium hydroxide, and Parker's "Super Quink" permanent blue-black ink to form a simple, inexpensive stain which produced fewer artifacts and less damage to the microscope.1 Aerosol OTB 1%, a product of American Cyanamid and Chemical Corporation, was used in the formula as a source of the dioctyl sodium sulfosuccinate. Their1 pharmacy stocked this material, but others may find that the wetting agent may be more easily secured from their pharmacies as a capsule intended for use as a laxative, "Dioctyll Capsules" (Person and Covey) or "Doxinate" (Lloyd Brothers). A 1% solution of the dioctyl sodium sulfosuccinate can be made by putting one capsule of the laxative in 25 cc of distilled water.
Edgar F. Luton, MD, Veterans Administration Hospital, Park Avenue and Getwell Street, Memphis, Tenn 38111.
Generic and Trade Names of Drugs
Dioctyl sodium sulfosuccinate—Colace, Doxinate.
LUTON EF. Substitution of Ingredient in Rapid Fungus Stain. Arch Dermatol. 1964;90(1):62. doi:10.1001/archderm.1964.01600010068014
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