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March 1965

Preparation of Permanent Stained Slide Cultures

Arch Dermatol. 1965;91(3):280-283. doi:10.1001/archderm.1965.01600090088015

THE USE OF the slide culture technique is well known among dermatologists as a valuable diagnostic and teaching aid in mycology. One of the disadvantages of this procedure has been the inability to maintain permanent stained mounts for future reference in a teaching program or when attempting to identify an unknown fungus. It is the purpose of this paper to describe a simple method of preparing permanent stained slide cultures.

Materials and Methods  The material needed is that used for the standard slide culture technique consisting of a Petri dish, glass tubing, microscope slide, cover glass, and agar.1

  1. The glass tubing is placed in the Petri dish and the glass slide is placed on it. The entire unit is then autoclaved. If an autoclave is not available, flaming will be satisfactory.

  2. A drop of melted agar is then pipetted on one end of the sterilized

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