The effects of light and hematoporphyrin on pooled human sera with elevated titers of antistreptolysin O, heterophil and "immune" anti-A antibodies, and high concentrations of rheumatoid factor were studied. In all experiments, the paper electrophoretic pattern of irradiated specimens containing porphyrin demonstrated a loss of definite globulin peaks and a decrease in albumins. Associated with this change was a reversal in the albumin/globulin (A/G) ratio in all specimens studied. Heterophil and immune anti-A antibodies were apparently very sensitive to light and hematoporphyrin, whereas the antistreptolysin O antibodies were not decreased. Hematoporphyrin caused nonspecific agglutination of latex particles. The combined action of light and porphyrins produced a striking and significant destruction of all three immune globulins.
BURNETT JW, SWANN BM, FIGGE FHJ. Effect of Light and Hematoporphyrin on Immunologic Components of Human Sera. Arch Dermatol. 1966;93(6):754–757. doi:10.1001/archderm.1966.01600240120024
Customize your JAMA Network experience by selecting one or more topics from the list below.