Mycobacterium leprae has been found in Khanolkar technique preparations to best advantage by combination with fluorescent staining.
AKERS WA, MORSE WC. Demonstration of Mycobacterium leprae in Tissue: Demonstration by Means of Khanolkar's Concentration Test Followed by Fluorescent Staining. Arch Dermatol. 1966;94(3):361–362. doi:10.1001/archderm.1966.01600270111024
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