A method is described for the differentiation of obligate and faculative anaerobic bacteria from skin using the replica plating technique. Anaerobic cultures containing both faculative and strict anaerobes are replicated by means of sterile velvet and the replicates incubated in air. By comparing the replicate plate with the original anaerobic plate, faculative anaerobes can be recognized and the remaining anaerobes identified.
McBride ME, Knox JM, Lively EM. Differentiation of Cutaneous Anaerobic Bacteria: The Use of the Replica Plating Technique. Arch Dermatol. 1967;96(3):296–297. doi:10.1001/archderm.1967.01610030074013
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