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January 1970

A Method for the Collection of Human Apocrine Sweat

Author Affiliations

San Francisco

From the Division of Dermatology, Department of Medicine, University of California School of Medicine, San Francisco.

Arch Dermatol. 1970;101(1):111. doi:10.1001/archderm.1970.04000010113022

The standard method for collecting human apocrine sweat as described by Hurley and Shelley1 employs the principle of capillary attraction. In obtaining significant quantities of apocrine sweat for chemical studies, we found the capillary tube method somewhat tedious in that the droplets were so small as to make their containment difficult. This note describes a simple modification of the capillary tube method that allows for quicker, more complete, and less tedious collection.

Our method uses vacuum suction to fill the capillary tube rather than capillary attraction.

Figures 1 and 2 depict the apparatus. A capillary tube of 0.8 mm internal diameter is bent at right angles for the collection of the sweat droplets. This tube is inserted through an airtight rubber stopper and held in a small cup with a tapered bottom. The cup is attached by 0.025 inch inside diameter plastic tubing to a vacuum pump.

In practice,

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