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September 1971

Direct Scanning Electron Microscopy Human Skin

Author Affiliations

New Brunswick, NJ

From Johnson & Johnson, New Brunswick, NJ.

Arch Dermatol. 1971;104(3):262-270. doi:10.1001/archderm.1971.04000210036007

Dermal and epidermal sheets obtained by sodium bromide separation of human skin may be directly examined with the scanning electron microscope. Unique views of four surfaces, the stratum corneum, basal cell layer, papillary dermis, and deeper cutis, as well as appendageal structures, are readily available. The simple technique utilized may be further modified by incubating the epidermal sheet in DOPA for selective study of melanocytes.