Dermatophyte test medium (DTM), obtained from two commercial sources, was compared with a Sabouraud agar medium containing antibiotics for the isolation of dermatologically significant fungi from clinical material. Dermatophyte test medium from both sources gave a smaller number of isolates of dermatophytes and Candida albicans than did the more conventional medium with which it was compared. These commercial DTMs cannot be recommended as utterly satisfactory routine isolation media where good laboratory facilities are available, but may be useful under less favorable clinical or field conditions.