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December 1971

Isoproterenol-Sensitive Adenyl Cyclase in a Particulate Fraction of Epidermis

Author Affiliations

Ann Arbor, Mich

From the Department of Dermatology, University of Michigan Medical School, Ann Arbor.

Arch Dermatol. 1971;104(6):601-610. doi:10.1001/archderm.1971.04000240025003

Adenyl cyclase was found in a 17,000 g particulate fraction of gently homogenized newborn rat epidermis. Isoproterenol produced a statistically significant 40% (P <.005) stimulation of this enzyme. We have previously shown a 205% stimulation of the enzyme by isoproterenol with intact epidermis from adult rats; thus it is apparent that the broken-cell preparation of epidermal adenyl cyclase has a decreased sensitivity to the hormone, which is characteristic of many adenyl cyclases from various tissues. Sodium fluoride, a stimulator of most adenyl cyclases, produced a 139% increase in enzyme activity. Propranolol, a beta-adrenergic antagonist, prevented the stimulation of epidermal adenyl cyclase by the beta-adrenergic agonist, isoproterenol. Thus the second criterion used to characterize a hormonally induced response, in this case the betaadrenergic inhibition of epidermal cell division, mediated by adenosine 3′,5′-monophosphate (cyclic AMP) appears to have been satisfied.

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