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April 1973

Antinuclear Antibodies-Reply

Arch Dermatol. 1973;107(4):631. doi:10.1001/archderm.1973.01620190095033

To the Editor.—  In response to Dr. Burnham's letter, we quote a portion of our paper1:Although paraffin-embedded sections can not be used to differentiate most ANA patterns, paraffin-embedded sections can be used for screening patients and titering sera, thus, eliminating the need to make multiple cryostat-cut sections.If the above screening test is positive, the more precise cryostat methods should be used to determine which of the four nuclear patterns are present; if the pattern has been determined, the screening test can be used for following titers.The paraffin-embedded sections can be purchased and stored until needed, so that any hospital or interested investigator can perform the test. The shelf-life of the paraffinembedded material is at least five years. The use of paraffin-embedded material eliminates the routine need for fresh tumors, or laboratory animals, that are needed for preparing cryostat sections or imprints, which deteriorate within weeks