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September 1973

Fluorescent Technique for Identification of Candida albicans From Skin Scrapings

Arch Dermatol. 1973;108(3):426. doi:10.1001/archderm.1973.01620240072029

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To the Editor.—  At times, differentiation of Candida from dermatophyte infection on potassium hydroxide preparations may be difficult, especially if only pseudomycelia are seen. While cultures may take only 24 to 48 hours to grow Candida albicans, often immediate diagnosis may be required. Immunofluorescent identification of C albicans from culture media has been available for several years (Sabouraudia 5:323, 1967). While identification of C albicans from direct immunofluorescence of vaginal secretions has been reported by Fribourg-Blanc (Ann Biol Clin 28: 269, 1970), we could find no reports of immunofluorescent studies for C albicans done on skin scrapings.

Materials and Methods  Seven consecutive patients with clinical candidiasis were examined. All had either budding yeast cells or pseudomycelia or both present in 10% potassium hydroxide preparations. All skin scrapings from these patients were subsequently shown to be culture positive for C albicans. Immunofluorescence of culture material was also shown to be positive for

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