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March 1974

Paired Comparison Vasoconstrictor Assays: A Comparison of Methods for the Determination of Relative Vasoconstrictor Potency of Topically Applied Corticosteroids

Author Affiliations

Palo Alto, Calif

From the Institute of Pharmaceutical Sciences (Dr. Poulsen), the Institute of Clinical Medicine (Dr. Burdick), and the Department of Biostatistics (Dr. Bessler), Syntex Research, Palo Alto, Calif.

Arch Dermatol. 1974;109(3):367-371. doi:10.1001/archderm.1974.01630030027005

The human vasoconstrictor assay, which is frequently used for potency comparisons of topically applied corticosteroids, relies on the visual measurement of skin blanching produced at the application site. In this report, three different methods of visual measurement were compared. A technique involving paired comparisons of vasoconstriction at adjacent sites was found to improve the assessment of relative vasoconstrictor activity. Corticosteroid creams containing 0.05% fluocinonide, 0.1% betamethasone valerate, and 1% hydrocortisone were evaluated in this study.

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