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May 1976

Green Autofluorescence in Human Epidermal Cells

Author Affiliations

From the Department of Dermatology, New York Medical College, Flower Fifth Avenue Hospital, and Bird S Coler Hospital, New York.

Arch Dermatol. 1976;112(5):667-670. doi:10.1001/archderm.1976.01630290017003

• A characteristic and natural autofluorescence that appears brilliant green in frozen sections of untreated skin when viewed under the fluorescent microscope is demonstrated. Study with various barrier filters and exciter filters indicates that the optimum absorption for visualization of this is 300 to 330 nm, and the optimum fluorescence is between 500 and 530 nm under the conditions used.

Clinical study of skin from 52 patients including black, white, Puerto Rican, and Chinese indicates a relationship between skin color and intensity of autofluorescence. In addition, the cellular localization of autofluorescence corresponds to sites of melanin. Cells in the basal layer are involved most conspicuously and nuclei are spared.

(Arch Dermatol 112:667-670, 1976)

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