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December 1976

Leukocyte Migration Inhibition Assay (LIF) in Nickel Contact Dermatitis

Author Affiliations

June Dvorak

From the Department of Dermatology, Medical College of Virginia, Richmond.

Arch Dermatol. 1976;112(12):1741-1744. doi:10.1001/archderm.1976.01630370029006

• A direct assay using the mixed leukocyte migration inhibition technique for leukocyte inhibition factor (LIF) production has shown that nickel-sensitive subjects can be distinguished from nonallergic controls. The difference between the two groups' migrating indexes was obtained with nickel sulfate in 199 medium at a concentration of 150 μg/ml and without prior coupling to a carrier protein. A concise review of the principal in vitro assays for cell-mediated immunity is included.

(Arch Dermatol 112:1741-1744, 1976)

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