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August 1978

Circulating Antibodies Reactive With Cytoplasmic Antigens of Upper and Germinal Cells of Human Skin

Arch Dermatol. 1978;114(8):1245-1246. doi:10.1001/archderm.1978.01640200093047

To the Editor.—  I read with great interest the article by Dr Bystryn entitled, "Clinical Significance of Basal Cell Layer Antibodies" (Archives 113:1380-1382, 1977). The author stated in this article that, based on previous reports, at least three types of epidermal cytoplasmic antibodies could be shown by indirect immunofluorescent (IIF) techniques. I would like to add some comments. In our experience of testing several sera in low dilutions (< 16), the IIF staining patterns (cytoplasmic antigens present in all keratinocytes, including the basal cells [G-CYT], cytoplasmic antigens present in the upper epidermis, excluding the basal cells [U-CYT], andComparison of Results of Indirect Immunofluorescent Techniques Performed on Serum-Containing BC-CYT Antibodies When Tested on Three Substrates* Guinea Pig Lip Substrate Animal 1 Animal 2 Animal 3 BC-CYT U-CYT BC-CYT U-CYT BC-CYT U-CYT Serum dilution 1:4 + + +(+) 1:16 (+) 1:32 +(+) 1:64 1:128With a low dilution of the serum (< 1:16), BC-CYT fluorescence in