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January 1986

LDA-1 Monoclonal Antibody: An Excellent Reagent for Immunofluorescence Mapping Studies in Patients With Epidermolysis Bullosa

Author Affiliations

From the Departments of Dermatology (Dr Fine) and Medicine (Dr Gay) and the LM-EM-Histochemistry Unit, Institute of Dental Research (Drs Fine and Gay), University of Alabama at Birmingham School of Medicine, and the Dermatology Service, Birmingham (Ala) Veterans Administration Medical Center (Dr Fine).

Arch Dermatol. 1986;122(1):48-51. doi:10.1001/archderm.1986.01660130052024

• To determine the diagnostic usefulness of a monoclonal antibody to the LDA-1 antigen, a newly defined noncollagenous component of the lamina densa of human basement membrane, we examined skin specimens from 20 patients with epidermolysis bullosa (simplex type, eight patients; junctional type, four patients; dystrophic type, eight patients) by the indirect immunofluorescence technique. We compared this antibody to polyclonal antibodies with three other antigens routinely examined in diagnostic immunofluorescence mapping studies. The antigen defined by the LDA-1 monoclonal antibody was expressed normally in all specimens examined, regardless of the type of epidermolysis bullosa present. Furthermore, no disparity was noted between the LDA-1 monoclonal antibody and a polyclonal antibody to type IV collagen in the microscopic localization of binding within induced skin blisters. These findings suggest that the LDA-1 monoclonal antibody is an excellent immunoreagent for diagnostic immunofluorescence mapping studies.

(Arch Dermatol 1986;122:48-51)

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