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July 1989

Special Techniques in Dermatology

Author Affiliations

From the Division of Dermatopathology, Department of Dermatology, University of Pennsylvania, Philadelphia.

Arch Dermatol. 1989;125(7):963-974. doi:10.1001/archderm.1989.01670190097014

• Beyond routine hematoxylin-eosin histologic examinations, specialized diagnostic techniques allow examination of biopsy material for subtle morphological and functional alterations. Morphological analytic techniques (1-μm section analysis, transmission electron microscopy, x-ray probe microanalysis, and digital image analysis) and functional analytic techniques (immunofluorescence, immunohistochemistry, and molecular biologic techniques) are valuable diagnostic tools. These techniques have applications in evaluating cutaneous T-cell lymphoma and atypical lymphocytic infiltrates, vesiculobullous disorders, lupus erythematosus and collagen vascular diseases, vasculitis, poorly differentiated tumors, storage diseases, and infections.

(Arch Dermatol. 1989;125:963-974)