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August 1989

Immune Response to ?-Irradiated Injectable Human Amnion and Human Skin Collagens in the Rat

Author Affiliations

From the Institute of Occupational Medicine, Chinese Academy of Preventive Medicine (Dr Liu), and the Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (Dr Xu), Beijing, China; and the Division of Plastic Surgery, Cora and Webb Mading Department of Surgery (Drs Harrell and Spira), and the Department of Biochemistry (Dr Dresden), Baylor College of Medicine, Houston, Tex.

Arch Dermatol. 1989;125(8):1084-1089. doi:10.1001/archderm.1989.01670200060009

• The immune response in rats to γ-irradiated human amnion and human skin collagen was characterized through histologic and immunologic methods. Pepsin-extracted human amnion collagen and skin collagen were purified and reconstituted. Implants of amnion collagen demonstrated greater persistence than skin collagen. For amnion collagen implants, no significant inflammatory response was found. Fibroblast and adipocyte ingrowth and neovascularization were present. Conversely, obvious inflammatory infiltration was evident in the skin collagen implants. Enzyme-linked immunosorbent assay results showed that anti-amnion collagen antibody levels were significantly lower than anti-skin collagen antibody levels against their respective implant materials. The ratios of type I to type III collagen are 56:44 and 95:5 for amnion collagen and skin collagen, respectively. These findings suggest that in this heterologous type system, type III collagen-rich amnion collagen preparations appear superior to skin collagen for softtissue augmentation.

(Arch Dermatol. 1989;125:1084-1089)

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