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December 1993

Detection of Human Immunodeficiency Virus Type 1 and Human Papillomavirus Type 16 in Acquired Immunodeficiency Syndrome-Related and Classic Kaposi's Sarcoma

Author Affiliations

Department of Dermatology Wayne State University Detroit, MI 48201

Pinkus Dermatopathology Laboratory Monroe, MI 48161

Arch Dermatol. 1993;129(12):1620-1622. doi:10.1001/archderm.1993.04540010098018

After the outburst of the acquired immunodeficiency syndrome (AIDS), AIDS-related Kaposi's sarcoma (AIDS-KS) has been commonly found among homosexual AIDS patients infected with human immunodeficiency virus type 1.1 Recently, a causative role of human papillomavirus (HPV) type 16 in KS was suggested, 2 although contradictory results were also reported.3 To elucidate the role of HIV-1 and HPV-16 in KS, we performed polymerase chain reaction-based analyses with DNA extracted from classic KS, AIDS-KS, and primary culture of AIDS-KS.

Observations.  DNA samples were extracted from formalin-fixed, paraffin-embedded tissue sections obtained from biopsy specimens from 15 classic KS cases from 1976 through 1979 and seven cases of AIDS-KS seen in 1991. Each section was cut with a disposable new razor blade and the epidermis was removed. Before the amplification of viral DNA, the integrity of each sample was checked with β-globin gene amplification, and negative samples of nine classic KS cases

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