Data are presented as predicted probabilities in each quartile (Q) adjusting for age, body mass index, smoking status, race, folate supplementation, organic fruit and vegetable consumption frequency, residential pesticide exposure history, total energy intake, Western and prudent pattern scores, and infertility diagnosis. The model for high–pesticide residue fruit and vegetable intake was additionally adjusted for low–pesticide residue fruit and vegetable intake and vice versa. Error bars indicate 95% confidence interval.
Data were adjusted for age, body mass index, smoking status, race, folate supplementation, organic fruit and vegetable consumption frequency, residential pesticide exposure history, total energy intake, Western and prudent pattern scores, and infertility diagnosis. Error bars indicate 95% confidence interval.
eTable 1. Adjusted probability of clinical pregnancy and live birth according to high pesticide fruit and vegetable intake, restricting for different characteristics
eTable 2. Ovarian biomarker and ovarian stimulation outcomes according to quartile of fruit and vegetable intake, considering pesticide residue status, among 305 women (424 fresh cycles)
eTable 3. Fertilization and embryo quality according to fruit and vegetable intake, considering pesticide residue status, in 305 women (424 fresh cycles) from EARTH study
eFigure. Overview of 541 initiated cycles in the EARTH Study between April 2007 and August 2016
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Chiu Y, Williams PL, Gillman MW, et al. Association Between Pesticide Residue Intake From Consumption of Fruits and Vegetables and Pregnancy Outcomes Among Women Undergoing Infertility Treatment With Assisted Reproductive Technology. JAMA Intern Med. 2018;178(1):17–26. doi:10.1001/jamainternmed.2017.5038
Is there an association between exposure to pesticide residues in fruits and vegetables and pregnancy outcomes?
In a cohort of 325 women undergoing infertility treatment with assisted reproductive technology, intake of high–pesticide residue fruits and vegetables was associated with a lower probability of live birth, while low–pesticide residue fruit and vegetable intake was not associated with this outcome.
Dietary pesticide exposure within the range of typical human exposure may be associated with adverse reproductive consequences.
Animal experiments suggest that ingestion of pesticide mixtures at environmentally relevant concentrations decreases the number of live-born offspring. Whether the same is true in humans is unknown.
To examine the association of preconception intake of pesticide residues in fruits and vegetables (FVs) with outcomes of infertility treatment with assisted reproductive technologies (ART).
Design, Setting, and Participants
This analysis included 325 women who completed a diet assessment and subsequently underwent 541 ART cycles in the Environment and Reproductive Health (EARTH) prospective cohort study (2007-2016) at a fertility center at a teaching hospital. We categorized FVs as having high or low pesticide residues using a validated method based on surveillance data from the US Department of Agriculture. Cluster-weighted generalized estimating equations were used to analyze associations of high– and low–pesticide residue FV intake with ART outcomes.
Main Outcomes and Measures
Adjusted probabilities of clinical pregnancy and live birth per treatment cycle.
In the 325 participants (mean [SD] age, 35.1 [4.0] y; body mass index, 24.1 [4.3]), mean (SD) intakes of high– and low–pesticide residue FVs were 1.7 (1.0) and 2.8 (1.6) servings/d, respectively. Greater intake of high–pesticide residue FVs was associated with a lower probability of clinical pregnancy and live birth. Compared with women in the lowest quartile of high-pesticide FV intake (<1.0 servings/d), women in the highest quartile (≥2.3 servings/d) had 18% (95% CI, 5%-30%) lower probability of clinical pregnancy and 26% (95% CI, 13%-37%) lower probability of live birth. Intake of low–pesticide residue FVs was not significantly related to ART outcomes.
Conclusions and Relevance
Higher consumption of high–pesticide residue FVs was associated with lower probabilities of pregnancy and live birth following infertility treatment with ART. These data suggest that dietary pesticide exposure within the range of typical human exposure may be associated with adverse reproductive consequences.
More than 90% of the US population has detectable concentrations of pesticides or their metabolites in their urine or blood samples.1 While pesticide exposure occurs through a variety of routes, the primary route in the general population is through diet–especially intake of conventionally grown fruits and vegetables (FVs).2-7 In the United States, pesticides are regulated and evaluated by the US Environmental Protection Agency to ensure the safety of the food supply for human consumption. Nonetheless, there has been a growing concern that permitted levels of pesticide residues in food defined by traditional toxicological testing may be too high, especially for susceptible populations such as pregnant women or infants.8,9
In rodent models, ingestion of pesticide mixtures in early pregnancy at a concentration assumed to be without adverse health effects increased the percentage of apoptosis in embryos and decreased the number of live pups born.10,11 Evidence from human studies is scarce. Women occupationally exposed to pesticides and women living in or near agricultural areas may have increased risk of infertility and adverse pregnancy outcomes.12-24 However, whether exposure within the range of typical human exposure, such as through diet, has any effect on reproductive outcomes in humans is unknown.
We previously developed and validated a low-cost, questionnaire-based method—the Pesticide Residue Burden Score (PRBS)—to estimate exposure to pesticide residues from FVs in epidemiologic studies.25-27 In the present study, we aimed to investigate the associations between preconception intake of high– and low–pesticide residue FVs and outcomes of assisted reproductive technologies (ART) in a prospective cohort of women undergoing infertility treatment.
Women in this study were participants in the Environment and Reproductive Health (EARTH) Study, an ongoing prospective cohort established in 2006 to identify determinants of fertility among couples presenting to the Massachusetts General Hospital Fertility Center (Boston, Massachusetts).28 Women were eligible to participate if they were between 18 and 45 years and planned to use their own gametes for infertility treatment. Women whose treating physician later determined that using donor eggs was clinically necessary remained in the study. Among women referred by physicians, approximately 60% of those approached by the research nurses enrolled in the study. Diet assessment was introduced to the study in 2007. The current analysis includes 325 women (contributing 541 ART cycles) whose diet was assessed and who contributed at least 1 subsequent ART cycle between April 2007 and August 2016. Women who did not complete a diet assessment (n = 113) or whose ART cycles started prior to assessment completion (n = 7) were excluded from the present analysis. The study was approved by the Human Studies Institutional Review Boards of the Massachusetts General Hospital, Harvard T.H. Chan School of Public Health, and the Centers for Disease Control and Prevention. All participants signed an informed consent after the study procedures were explained by trained study staff.
On entry, height and weight were measured by trained study staff to calculate body mass index (BMI, calculated as weight in kilograms divided by height in meters squared). Study staff also administered a brief questionnaire to collect data on demographic characteristics, medical history, and lifestyle factors. Participants completed a detailed take-home questionnaire with additional questions on reproductive history and lifestyle factors. On this take-home questionnaire, participants were asked how often they consumed organic FVs during the past 3 months. We considered women to be organic FV consumers if they consumed organic FVs at least 3 times per week (the median in this population); women with lower intake of organic FVs (<3 times/wk) were considered to be conventional FV consumers.
Clinical information was abstracted by trained study staff from the patients’ electronic medical records. We have previously described details of patient clinical management elsewhere.28 Briefly, clinical staff monitored patients during gonadotropin stimulation for serum estradiol, follicle size and counts, and endometrial thickness for 2 days before oocyte retrieval, and administered human chorionic gonadotropin (β-hCG) to induce ovulation approximately 36 hours before oocyte retrieval. Embryologists classified oocytes as germinal vesicle, metaphase I, metaphase II (MII), or degenerated, and determined fertilization rate as the number of oocytes with 2 pronuclei divided by the number of MII oocytes at 17 to 20 hours after insemination. Cell cleavage rates of embryos were considered to be normal with a division of 2 to 4 cells on day 2 and 4 to 8 cells on day 3 of culture. A division below 2 cells on day 2 and 6 cells on day 3 was considered as slow while a division of 4 or more cells on day 2 and 8 or more cells on day 3 was designated accelerated. For this study, early ART end points referred to any end points prior to embryo transfer, including markers of ovarian responses to stimulation (peak estradiol levels, endometrial thickness, MII and total oocytes), fertilization rate, and embryo quality. We excluded egg donor and cryogenic cycles for the analysis of early ART end points.
Clinical outcomes were assessed per initiated cycle, including implantation (defined as a serum β-hCG level >6 mIU/mL [to convert to IU/L, multiply by 1.0] typically measured approximately 17 days after oocyte retrieval), clinical pregnancy (defined as presence of intrauterine gestational sac[s] on ultrasonography at 6 weeks), and live birth (as the birth of a neonate on or after 24 weeks of gestation).
We categorized total pregnancy loss into (1) early pregnancy loss, defined as a positive urine β-hCG test followed by the absence of signs of clinical pregnancy, including chemical pregnancy loss and ectopic pregnancy; and (2) clinical pregnancy loss, defined as an intrauterine pregnancy demise after a clinical pregnancy, including spontaneous abortion, stillbirth, and therapeutic abortion. No molar pregnancies occurred in this cohort.
Diet was assessed before initiation of ART using a self-administered, previously validated food frequency questionnaire.29 Women reported how often they typically consumed specified amounts of each food, beverage, and supplement over the past year. Two data-derived dietary pattern scores, the prudent and Western pattern,30 were used to summarize overall food choices.
We used the annual reports from the US Department of Agriculture Pesticide Data Program (PDP) to classify FVs according to their mean pesticide residue status in the US food supply.31 Details of the PRBS methods have been described elsewhere.25,27,32 We considered 3 measures of contamination from the PDP to classify FVs: (1) the percentage of samples tested with any detectable pesticides, (2) the percentage of samples tested with pesticides exceeding the tolerance level, and (3) the percentage of samples with 3 or more individual detectable pesticides. The pesticide residue data in FVs were averaged by annual PDP reports from 2006 through 2015, corresponding to the periods when the diet history of the participants was captured by the food frequency questionnaire.
Next, we categorized foods according to tertiles for each of the 3 measurements of contamination and assigned a score of 0 to FVs in the bottom tertile, 1 to FVs in the middle tertile, and 2 for FVs in the top tertile. The PRBS for each food was the sum of scores across the 3 PDP contamination measures. We considered FVs with a PRBS of 4 or greater on a scale of 0 to 6 to be high–pesticide residue foods while FVs with a PRBS of less than 4 to be low–pesticide residue foods. Based on these criteria, 14 FVs were categorized as high pesticide residue and 22 as low pesticide residue (Table 1).
Women were classified according to quartiles of total FV intake, high–pesticide residue FV intake, and low–pesticide residue FV intake. We conducted Kruskal-Wallis tests (for continuous variables) and Fisher exact tests (for categorical variables) to compare baseline characteristics across quartiles of FV intake. To evaluate the relationship of FV intake with ART outcomes, we used cluster-weighted generalized estimating equations to account for within-person correlations in the presence of nonignorable cluster size.33 Each observation was weighted inversely to the number of cycles they contributed to the analysis. We evaluated ART outcomes per initiated cycle to estimate effects relevant in practice and mirror intention-to-treat analyses for studies of ART.34,35 However, in a post-hoc analysis, we evaluated the association of FV intake with risk of pregnancy loss only among cycles in which implantation was achieved.34 Population marginal means were used to present population averages adjusted for the covariates at their average levels for continuous variables and weighted average levels of categorical variables in the model.36 Tests for linear trend were performed using the median intake of FVs in each quartile as a continuous variable.
Confounding was evaluated using directed acyclic graphs based on prior knowledge.
Specifically, variables previously reported to be associated with live birth/pregnancy loss as well as associated with FV intake were considered as potential confounders.37-40 In addition, we included dietary pattern scores to distinguish relations between FV intake from those of overall food choices. The final multivariable models were adjusted for age (years), BMI, smoking status (current/former vs never), race (white vs nonwhite), supplemental folate intake (micrograms per day), organic FV consumption frequency (<3 vs ≥3 times/wk), residential pesticide exposure history (yes vs no), prudent and Western dietary patterns, total energy intake (kilocalories per day), and infertility diagnosis (male factor vs female factor vs unexplained). The model for high–pesticide residue FV intake was additionally adjusted for low-pesticide FV intake and vice versa because they may confound each other. To minimize residual confounding, we performed separate sensitivity analyses restricting to women younger than 40 years, women without a history of miscarriage, autologous cycles, and cycles initiated within 1 year of food frequency questionnaire completion. We also estimated the effect of substituting 1 serving/d of low–pesticide residue FVs for high–pesticide residue FVs on clinical outcomes.41 All statistical analyses were performed in SAS, version 9.4 (SAS Institute). P values were 2 sided. Findings were considered statistically significant when P < .05.
A total of 325 women underwent 541 ART cycles (range, 1-6), of which 228 (42%) resulted in a live birth (eFigure 1 in the Supplement). Women had a mean (SD) intake of 1.7 (1.0) servings/d of high–pesticide residue FVs and 2.8 (1.6) servings/d of low–pesticide residue FVs. Intakes of high– and low–pesticide residue FVs were positively correlated with each other (Spearman r = 0.57). Women who consumed more high–pesticide residue FVs were more likely to report regular organic FV consumption, had higher total calorie and micronutrient intake, higher adherence to the prudent dietary pattern, and a slightly higher prevalence of diminished ovarian reserve. Similar trends were observed with greater intake of low–pesticide residue FVs except that no difference in prevalence of diminished ovarian reserve was observed. Other characteristics were similar across quartiles of high- or low-pesticide FV intake (Table 2).
Total FV intake was unrelated to probability of implantation, clinical pregnancy, and live birth (Table 3). However, when FVs were classified as having high or low pesticide residues, divergent patterns of associations with clinical pregnancy and live birth emerged (Table 3). Specifically, high–pesticide residue FV intake was inversely associated with probability of clinical pregnancy and live birth per initiated cycle. Compared with women in the lowest quartile of high–pesticide residue FV intake (<1 serving/d), women in the highest quartile (≥2.3 servings/d) had 18% (95% CI, 5%-30%) lower probability of clinical pregnancy and 26% (95% CI, 13%-37%) lower probability of live birth. These associations persisted in sensitivity analyses (eTable 1 in the Supplement). Low–pesticide residue FV intake was associated with a higher, albeit nonsignificant, probability of clinical pregnancy and live birth (Table 3). We found no associations between intake of high– or low–pesticide residue FVs with markers of response to ovarian stimulation, fertilization rate, or embryo quality (eTable 2 and eTable 3 in the Supplement).
Next, we examined the associations of FV intake with risks of pregnancy loss (Figure 1). High–pesticide residue FV intake was positively associated with probability of total pregnancy loss. The adjusted probabilities of total pregnancy loss were 7% (95% CI, 3%-15%), 23% (95% CI, 16%-33%), 24% (95% CI, 15%-36%), and 34% (95% CI, 20%-51%) for women in increasing quartiles of high–pesticide residue FV intake (P = .04 for trend). When total pregnancy loss was divided into early and clinical pregnancy loss, the trends were similar. On the other hand, low–pesticide residue FV intake was inversely associated with early pregnancy loss but unrelated to clinical pregnancy loss.
Last, we estimated the effect of replacing high–pesticide residue FVs with low–pesticide residue FVs on the odds of clinical outcomes (Figure 2). Consuming 1 serving/d of low–pesticide residue FVs in lieu of 1 serving/d of high–pesticide residue FVs was associated with 79% (95% CI, 11%-188%) higher odds of clinical pregnancy and 88% (95% CI, 16%-205%) higher odds of live birth.
We evaluated the association between preconception intake of FVs, considering their pesticide residue status, and ART outcomes among women undergoing infertility treatment. We observed that greater intake of high–pesticide residue FVs was associated with lower probabilities of clinical pregnancy and live birth per initiated cycle. The observed association with live births was driven by a higher risk of early and clinical pregnancy loss. On the other hand, low–pesticide residue FV intake was associated with a lower risk of early pregnancy loss. Replacing high–pesticide residue FVs with low–pesticide residue FVs was estimated to provide the greatest benefit for achieving clinical pregnancy and live birth.
While FVs are an important part of a healthy diet,42 they also serve as the primary vehicle for pesticide residue exposure in the general population.3 Earlier studies have shown that many pesticides used in agriculture have deleterious effects on reproductive health outcomes, such as decreased fertility, spontaneous abortion, stillbirth, or developmental abnormalities,12-24 while a few others reported no associations.43,44 Of note, in one of these studies, among 684 participants (73 cases, 611 controls) from agricultural counties of California, Bell et al18 found that the adjusted odds ratio of fetal death for those exposed to 3 or more pesticide classes was 2.6 (95% CI, 1.3-5.3), while those exposed to 1 or 2 pesticide classes had an odds ratio of 1.1 (95% CI, 0.6-2.1). In another study of women living on Ontario farms, Arbuckle et al24 showed that exposure to both fungicides and herbicides before conception doubled the risk of spontaneous abortion relative to women exposed only to fungicides, suggesting that pesticide mixtures may confer a greater risk of fetal loss. Nonetheless, most of these studies have focused on occupational workers or women living in or near agricultural areas. The influence of exposure to pesticide residues primarily through foods on pregnancy outcomes in the general population remains unknown.
To the best of our knowledge, this is the first prospective study evaluating the relationship of dietary pesticide exposure to reproductive success in humans. The most closely related study to ours is a prospective study of 28 192 Norwegian women, which found that women choosing organically grown vegetables during pregnancy had reduced risk of preeclampsia regardless of adjustment for various healthy food scores.45 One possible explanation was that organic vegetable consumption may reduce exposure to pesticides. Some forms of miscarriage and preeclampsia may be related, representing a continuum whose origin is oxidative stress–induced placental dysfunction.46,47 Pesticide-induced placental dysfunction46,48,49 may explain the relationship of lower rates of clinical pregnancy loss associated with lower intake of high–pesticides FVs in the present study, as well as lower prevalence of preeclampsia associated with organic vegetable consumption in the earlier study.45 However, given the paucity of the data, future studies are warranted to replicate these findings.
Our results are also in agreement with experimental animal data. Cavieres et al11 showed that pregnant mice exposed to a pesticide mixture at a level lower than drinking water standards during a period spanning preimplantation and organogenesis produced a significant decrease in implantation sites and number of live pups born. Further, Greenlee and colleagues10 showed that a mixture of agricultural chemicals at 1 reference dose (ie, an estimate of daily oral exposure that is likely to be without an appreciable risk of deleterious effects during lifetime) increased blastomere apoptosis and suppressed cell proliferation of morulae, which may result in embryonic demise or pregnancy loss. It is possible that pesticides may impair pregnancy maintenance by affecting peri-implantation embryo development, which is known as a period of heightened susceptibility to malformations.50(pp421-423)
Our study has some limitations. First, exposure to pesticides was not directly assessed but was rather estimated from self-reported FV intake paired with pesticide residue surveillance data. Although we have adjusted for organic FV intake, data on whether individual FVs were consumed as organic or conventional were not collected, possibly leading to exposure misclassification. However, our previous work has shown that higher intake of high–pesticide residue FVs was significantly associated with higher levels of urinary pesticide metabolites, supporting the use of the PRBS as an adequate measure to characterize exposure to pesticides through diet.26 Second, our methodology does not allow linking specific pesticides to adverse reproductive effects. Further confirmation studies, preferably accounting for common chemical mixtures used in agriculture by biomarkers, are needed. Third, as in all observational studies, we cannot rule out the possibility that residual (eg, significant differences in organic FV consumption across quartiles of high–pesticide residue FV intake) or unmeasured confounding may still be explaining some of our observed associations. However, women with greater high-pesticide FV intake and those of greater low-pesticide FV intake had similar patterns of baseline characteristics, suggesting that the observed associations are due to intake of pesticide residues rather than to residual confounding. Furthermore, results were consistent after accounting for many factors that could potentially affect the risk of pregnancy loss. An additional limitation is that findings may not be generalizable to the general population because participants were recruited through a fertility clinic and intake of FVs in our cohort was double the median intake in the US population (2 servings/d).51 However, the infertility cohort allowed us to examine the association of dietary pesticide exposure with many pregnancy outcomes that are not observable among couples becoming pregnant on their own such as very early pregnancy losses. In addition, demographic characteristics of the study participants were comparable to those of women seeking fertility treatment in the United States,52 suggesting that results may be generalizable to women seeking infertility treatment. Additional strengths of the study include its prospective study design and well-documented outcome measures.
In conclusion, intake of high–pesticide residue FVs was associated with lower probabilities of clinical pregnancy and live birth among women undergoing infertility treatment. Our findings are consistent with animal studies showing that low-dose pesticide ingestion may exert an adverse impact on sustaining pregnancy.11 Because, to our knowledge, this is the first report of this relationship in humans, confirmation of these findings is warranted.
Corresponding Author: Jorge E. Chavarro, MD, ScD, 665 Huntington Ave, Boston, MA 02115 (firstname.lastname@example.org).
Accepted for Publication: August 22, 2017.
Published Online: October 30, 2017. doi:10.1001/jamainternmed.2017.5038
Author Contributions: Drs Chiu and Chavarro had full access to all of the data in the study and take responsibility for the integrity of the data and the accuracy of the data analysis.
Study concept and design: Chiu, Williams, Hauser, Chavarro.
Acquisition, analysis, or interpretation of data: All authors.
Drafting of the manuscript: Chiu, Gaskins, Chavarro.
Critical revision of the manuscript for important intellectual content: All authors.
Statistical analysis: Chiu, Williams, Gaskins, Mínguez-Alarcón, Chavarro.
Obtained funding: Hauser, Chavarro.
Administrative, technical, or material support: Gaskins, Toth, Ford.
Supervision: Williams, Gillman, Souter, Hauser, Chavarro.
Conflict of Interest Disclosures: None reported.
Funding/Support: The present work was supported by grants R01ES022955, R01ES009718, and P01ES000002 from the National Institute of Environmental Health Sciences (NIEHS) and grant P30DK046200 from the National Institute of Diabetes and Digestive and Kidney Diseases. Dr Gaskins was supported by K99ES026648 from the NIEHS.
Role of the Funder/Sponsor: The funders had no role in the design and conduct of the study; collection, management, analysis, and interpretation of the data; preparation, review, or approval of the manuscript; and decision to submit the manuscript for publication.
Group Information: The EARTH Study Team are Charles Bormann, PhD HCLD, Massachusetts General Hospital; Courtney Carignan, PhD, Yu-Han Chiu, MD, MPH, ScD, Georgios Christou, MD, Ramace Dadd, BA, Jennifer B. Ford, RN, BSN, Tamarra James-Todd, MPH, PhD, Myra G. Keller, RNC, BSN, Carmen Messerlian, PhD, Lidia Mínguez-Alarcón, PhD, Feiby Nassan, ScD, and Paige Williams, MS, PhD, Harvard T.H. Chan School of Public Health; Jorge Chavarro, MD, ScM, ScD, and Audrey J. Gaskins, ScD, Harvard T.H. Chan School of Public Health and Brigham and Women’s Hospital, Harvard Medical School; Russ Hauser, MD, ScD, MPH, Harvard T.H. Chan School of Public Health and Massachusetts General Hospital, Harvard Medical School; John Petrozza, MD, Mary Sabatini, MD, PhD, Jan Shifren, MD, Irene Souter, MD, Cigdem Tanrikut, MD, and Thomas L. Toth, MD, Massachusetts General Hospital and Harvard Medical School.
Disclaimer: The views expressed in this article do not necessarily represent the views of the US government, the Department of Health and Human Services, or the National Institutes of Health.
Additional Contributions: The authors gratefully acknowledge the contributions of physicians and staff at the Massachusetts General Hospital fertility center. A special thank you is due to all of the study participants.
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