A method is presented for determining whole blood volume by means of the circulating corpuscle volume. After a transfusion of unlike group blood, the transfused corpuscles can be separated from the recipient's corpuscles by agglutinating the latter with a serum of the same type as that of the blood transfused. If the transfusion is of blood of Group 1 (Group 4, Moss) into a recipient of Group 2, 3 or 4 (Group 1, Moss), the separation will be nearly quantitative. Since the amount of blood transfused is known, the degree of its dilution in the recipient may be determined and the blood volume calculated.
The method, which has already been noted,1 is of interest because, owing to the simplicity of the procedure and to the stability of the unlike group blood in the circulation of the recipient, multiple determinations can be made over a considerable length of time. The method
ASHBY W. BLOOD VOLUME: I. A METHOD FOR DETERMINING WHOLE BLOOD VOLUME BASED ON THE CIRCULATING CORPUSCLE VOLUME. Arch Intern Med (Chic). 1925;35(4):516–526. doi:https://doi.org/10.1001/archinte.1925.00120100114010
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