Kitzmiller and I have certain new developments in technic to offer those who may be interested in our antithrombin test previously described.1 These are, first, the use of purified fibrinogen and thrombin solutions instead of citrated horse plasma and serum; second, the adaptation of the method to from 0.1 to 0.2 cc. of the patient's serum so that venipuncture may be dispensed with; and, third, such simplification of the method that laboratory technicians anywhere may perform it, without possessing special knowledge of blood clotting.
The first alteration in technic, the use of purified fibrinogen and thrombin solutions, was prompted by the discovery that repeated bleedings of an animal to obtain the citrated plasma needed caused the development of such an excess of antithrombin in the animal's blood as to render it an unfit medium for detecting an excess of this element in a patient's blood. By the use of the
MILLS CA. ANTITHROMBIN TEST IN TYPHOID FEVER: IMPROVEMENTS IN TECHNIC. Arch Intern Med (Chic). 1927;39(5):618–620. doi:10.1001/archinte.1927.00130050015002
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