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Article
November 1927

MATERIAL FROM LYMPH NODES OF MAN: II. STUDIES ON LIVING AND FIXED CELLS WITHDRAWN FROM LYMPH NODES OF MAN

Author Affiliations

BOSTON

From the Medical Service of the Collis P. Huntington Memorial Hospital of Harvard University.

Arch Intern Med (Chic). 1927;40(5):647-NP. doi:10.1001/archinte.1927.00130110075007
Abstract

In a previous paper I described a simple method for obtaining living cells from lymph nodes or tumors.

Particular stress has been placed on the study of living cells, because one not only obtains from them a true cytologic picture, but also can observe them in their physiologic behavior to altered environmental conditions. Apparently most of the work on the pathology of lymph nodes has been concerned chiefly with their altered architecture and their grosser histology. Dr. George R. Minot suggested to me that a study of the fresh cells of lymph nodes might be worth while, and from former work I believed that a closer scrutiny of cell organs, such as mitochondria specific granules, segregation bodies and other cytoplasmic and nuclear structures, not only in fixed preparations, but also with special reference to the pathologic physiology and histology of these organs in living cells, might provide valuable information. It

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