Castle and Taylor1 have recently shown that maximal responses of reticulocytes were obtained in the treatment of patients with pernicious anemia, during relapse, when the amount of liver extract derived from 100 Gm. of liver was administered intravenously. The solution suitable for intravenous injection was prepared by dissolving approximately 4.5 Gm. of a commercial preparation of fraction G2 (liver extract) in physiologic solution of sodium chloride after washing the extract in ether. The solution was then filtered or centrifugated, sterilized by boiling for five minutes and made so that 20 cc. contained the amount of material derived from 100 Gm. of whole liver.
In studying the nature of the active principle in liver, Cohn3 and West4 and their respective associates had previously prepared highly purified fractions of liver which were suitable for intravenous injection and which were found to be potent. The practical application of these
FOUTS PJ, ZERFAS LG. USE OF LIVER EXTRACT INTRAVENOUSLY: REPORT OF TEN CASES. Arch Intern Med (Chic). 1932;50(1):27–36. doi:10.1001/archinte.1932.00150140034002
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