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Article
May 1938

DIRECT MEASUREMENT OF HEIGHT OF THYROID EPITHELIUM: A METHOD OF ASSAY OF THYROTROPIC SUBSTANCE; CLINICAL APPLICATION

Author Affiliations

CHICAGO

From the Department of Medicine, Northwestern University Medical School.

Arch Intern Med (Chic). 1938;61(5):726-738. doi:10.1001/archinte.1938.00180100036005
Abstract

A convenient, specific and delicate method of assay of the thyrotropic principle of the anterior lobe of the pituitary body is required for physiologic and clinical studies. Various methods have been used. Loeb1 and his associates, in 1928, described a quantitative method that had been used by them for several years, namely, the determination of the mitotic index. The entire thyroid gland of the test animal, the guinea pig, was cut in serial section and stained with hematoxylin and eosin. The average number of sections obtained for each gland varied between four hundred and fifty and five hundred; mitoses were counted in every tenth section in an exact manner; the number of mitoses thus obtained was multiplied by 10 to obtain the total number of mitoses in the gland. A marked increase in the number of mitoses occurs with thyroid hypertrophy.

Several workers have described the degrees of hyperplastic

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