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Bensley and Bensley have prepared a comprehensive manual of laboratory methods for the histologic worker which should prove valuable.
In the section on technics for the study of fresh tissues the authors discuss supravital stains, microdissection, tissue transplants into the anterior chamber of the eye and the Altmann-Gersh freezing-drying method.
They present not only all the older standard technics for the preparation and staining of fixed tissues but more recently introduced methods, such as the use of dioxane (diethylene dioxide) in dehydration. The book includes a section on the care of the microtome, sharpening of the knife and maintenance of laboratory equipment. A copy of this handbook should be in every histologic laboratory.