One of the earliest attempts to estimate chemically the concentration of adrenal cortex steroids in blood was made by Hemphill and Reiss, who measured the total reducing ability of blood-lipid extracts by means of the Hagedorn-Jensen blood sugar method.1 In 1948, Corcoran and Page2 applied the procedure for the measurement of formaldehydegenic steroids to extracts of blood plasma. Recent chemical methods have provided a more specific means for the measurement of adrenocortical substances with a 17-hydroxy-ketol side-chain, such as occur in compounds E (cortisone), F (hydrocortisone), and S.
In 1952, Nelson and Samuels 3 reported a method for the direct quantitation of 17-hydroxy-adrenocortical steroids. According to their procedure, the blood extract was purified by solvent partition and chromatography on a synthetic magnesium silicate (Florisil) column. The 17-hydroxy blood corticosteroids were estimated by a micromodification of the phenylhydrazine reaction as introduced by Porter and Silber,4 which is stated
FREEMAN S, WHEELER JX, HOEGEMEIER HW. Free Reducing and Hydrocortisone-like Steroids in Human Plasma: Study on Healthy and Diseased Subjects. AMA Arch Intern Med. 1956;97(1):45–50. doi:10.1001/archinte.1956.00250190061003
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