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February 1958

Bone Marrow Hemosiderin and Ferrokinetics Patterns in Anemia: I. Pernicious Anemia

Author Affiliations

San Francisco

From the Children's Hospital Research Laboratory, San Francisco, and the Donner Laboratory, University of California, Berkeley, Calif.

AMA Arch Intern Med. 1958;101(2):418-424. doi:10.1001/archinte.1958.00260140250035

Increased tissue iron in patients with pernicious anemia was first described in 1910, by Ryffel,1 who used autopsy material. Increased hemosiderin in bone marrow aspirates was demonstrated in 1948, by Rath and Finch,2 who showed that such a rise in bone marrow hemosiderin is common to all anemias except that of iron deficiency and that it is due largely to a shift of iron from the red blood cells to storage.3 Beutler recently reviewed quantitative aspects of iron stores.4 The present study of marrow hemosiderin deals with its pattern of distribution rather than its total amount. Whenever possible observations on its microscopic appearance were correlated with data obtained from ferrokinetic measurements.

Methods and Material  Bone marrow aspirations were performed using a 16 gauge needle* and a 10 ml. syringe previously rinsed with a 10% solution of edathamil disodium (Disodium Versenate). The aspirate was ejected on an