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April 1960

Studies on Ultrafiltrable Calcium

Author Affiliations

Shiraz, Iran

From the Department of Medicine, University Hospital, Minneapolis, Minnesota.

AMA Arch Intern Med. 1960;105(4):560-573. doi:10.1001/archinte.1960.00270160058008

Pribam in 1871 (cited by Loeb)35 first suggested that calcium exists in blood in more than one form. However, Rona and Takahashi51,52 in 1911, by using compensatory dialysis, showed for the first time the existence of blood calcium in dialyzable and nondialyzable forms and suggested that the latter was bound to protein. They found 75% of the total calcium to be diffusible.

Many papers 3,4,25-29,34-36,38,54,57,60,61 were published since then and many ideas were expressed regarding the different forms in which calcium existed, but without definite conclusions until 1934 when McLean and Hastings 40-43 performed some classical experiments by using the "frog's-heart" technique. They found 40% to 50% of the total calcium as ionic and an insignificant amount of diffusible calcium as reversibly bound to citrate. The nondiffusible calcium was accounted for by the presence of calciumprotein complex. The ionization of calciumproteinate was determined by an equilibrium between calcium