The emphasis in recent years on "significant bacteriuria" 1 has led to the recognition that urine cultures and colony counts are the best laboratory procedures available to the clinician in ascertaining the pressure of urinary tract infections. Kass 1 and others have shown significant urinary infection is present if two consecutive urine specimens, appropriately collected, contain more than 100,000 colonies per milliliter of bacteria. Further, when bacterial counts are between 10,000 and 100,000 colonies per milliliter, additional specimens should be tested since they often are found to have more than 100,000 colonies per milliliter.1,3 The Gram-stained preparation of a drop of fresh, unspun urine sediment and a microscopic examination of a drop of fresh, well-mixed urine have been also advocated as excellent, simple procedures for the detection of infected urine. Kass2 has claimed an 80% correlation between the presence of bacteria on the Gram stain and colony counts
BULGER RJ, KIRBY WMM. Simple Tests for Significant Bacteriuria. Arch Intern Med. 1963;112(5):742–746. doi:10.1001/archinte.1963.03860050129015
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