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November 1970

Bone Formation and Resorption in Tissue Culture: Effect of Agents Implicated in Renal Osteodystrophy

Author Affiliations

Rochester, NY

From the departments of pharmacology, toxicology, and medicine, University of Rochester (NY) School of Medicine and Dentistry.

Arch Intern Med. 1970;126(5):887-890. doi:10.1001/archinte.1970.00310110157028

The effects of many factors which are implicated in the pathogenesis of azotemic renal osteodystrophy can be separately analyzed and their interactions can be studied in tissue cultures of embryonic bone. Parathyroid hormone (PTH) and the active metabolite of vitamin D, 25-hydroxycholecalciferol (HCC) are both direct stimulators of bone resorption and act synergistically. Deficiency of HCC may explain the relative resistance to high levels of PTH in uremia. Different patterns of inhibition of bone resorption can be distinguished in tissue culture with such agents as phosphate, thyrocalcitonin (TCT), and dactinomycin. Tissue cultures can also be used to demonstrate effects on bone growth which is inhibited by PTH and stimulated by phosphate. These methods can serve as models for the study of other factors implicated in uremic bone disease.

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