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June 1976

Lymphocyte Blastogenesis in Patients Receiving Hemodialysis

Author Affiliations

From the Department of Nephrology, Walter Reed Army Institute of Research, Washington, DC (Drs Webel, Briggs, and Light), and the Department of Microbiology, Mayo Clinic and Mayo Foundation, Rochester, Minn (Dr Ritts). Dr Webel is currently with the Southern Illinois University School of Medicine, Carbondale, Ill. Read in part before the Southern Sectional meeting, American Federation of Clinical Research, New Orleans, Jan 24, 1974.

Arch Intern Med. 1976;136(6):682-687. doi:10.1001/archinte.1976.03630060034008

Lymphocyte blastogenic transformation in response to plant lectins and allogenic cells was studied in patients with nonuremic, far-advanced, chronic renal failure and in healthy controls. Cell cultures were studied in the presence of normal sera, patient's sera, and with media of different buffering capacities. Minimal blastogenic depression was observed when patient's lymphocytes were cultured in indifferent plasma with effective bicarbonate buffering compared with the use of pooled patient's plasma or HEPES buffer. Fresh plasma in culture depressed concanavalin A (Con A) blastogenesis. The data suggest that, under optimal conditions, lymphocytes from patients with chronic severe renal insufficiency are more responsive to stimuli than previously reported and as a group are near normal control values. Further, the defect observed may be a result of intracellular acidosis.

(Arch Intern Med 136:682-687, 1976)

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