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Article
May 1909

A METHOD FOR HEMOLYSIS AND AGGLUTINATION TESTS

Arch Intern Med (Chic). 1909;III(4):286-288. doi:10.1001/archinte.1909.00050150011002

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Abstract

The established methods of testing for agglutination and hemolysis have been developed chiefly in animal experimentation, and their application to work with human blood is encumbered with certain hindrances. In all serum reactions there is great need for control tests. The quantity of blood which it is necessary to take from patients for these tests limits the work to a certain extent and, therefore, interferes with the drawing of definite conclusions.

To overcome these obstacles we have developed a method whereby it is possible to perform a large number of tests with a very small amount of blood. In principle the method is identical with that now used by all investigators in this field. In details of technic it is an application of the method used by Wright in his work with opsonins. It has for its main object the reduction to a minimum of the amount of

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