Analytic methods depending on the optical properties of a finely divided suspension have recently achieved considerable prominence. Both the turbidimeter and the nephelometer have been used successfully in quantitative work when the amounts of material were too small to weigh or when the recovery of the substance to be weighed demanded long and tedious manipulation. The quantitative determination of albumin in urine presents many of these difficulties. This fact was pointed out by Folin and Denis1 in a paper published in 1914, in which a method was described whereby a quantitative estimation of protein in urine could be performed rapidly by means of the turbidimeter. The results obtained agreed well with gravimetric determinations. Since a method depending on the amount of light reflected by a turbid liquid should permit of the determination of smaller quantities of material than one in which the absorbed light is measured, it was
MARSHALL JTW, BANKS HW, GRAVES SS. A STUDY OF PROTEINS IN URINEAND A COMPARISON OF GRAVIMETRIC AND NEPHELOMETRIC METHODS FOR THEIR ESTIMATION. Arch Intern Med (Chic). 1916;XVIII(2):250–262. doi:10.1001/archinte.1916.00080150115007
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