Epidemiological evidence suggests blood pressure–lowering effects of cocoa and tea. We undertook a meta-analysis of randomized controlled trials to determine changes in systolic and diastolic blood pressure due to the intake of cocoa products or black and green tea.
MEDLINE, EMBASE, SCOPUS, Science Citation Index, and the Cochrane Controlled Trials Register were searched from 1966 until October 2006 for studies in parallel group or crossover design involving 10 or more adults in whom blood pressure was assessed before and after receiving cocoa products or black or green tea for at least 7 days.
Five randomized controlled studies of cocoa administration involving a total of 173 subjects with a median duration of 2 weeks were included. After the cocoa diets, the pooled mean systolic and diastolic blood pressure were −4.7 mm Hg (95% confidence interval [CI], −7.6 to −1.8 mm Hg; P = .002) and −2.8 mm Hg (95% CI, −4.8 to −0.8 mm Hg; P = .006) lower, respectively, compared with the cocoa-free controls. Five studies of tea consumption involving a total of 343 subjects with a median duration of 4 weeks were selected. The tea intake had no significant effects on blood pressure. The estimated pooled changes were 0.4 mm Hg (95% CI, −1.3 to 2.2 mm Hg; P = .63) in systolic and −0.6 mm Hg (95% CI, −1.5 to 0.4 mm Hg; P = .38) in diastolic blood pressure compared with controls.
Current randomized dietary studies indicate that consumption of foods rich in cocoa may reduce blood pressure, while tea intake appears to have no effect.
An increased consumption of fruits and vegetables is recommended as a first-line therapeutic approach in current hypertension control guidelines.1,2 At least part of the reduction of blood pressure and lowering cardiovascular risk has been attributed to the polyphenols (flavonoids) in fruits and vegetables.3-5 Tea and cocoa products account for the major proportion of total polyphenol intake in Western countries.6,7 However, cocoa or tea are currently not implemented in cardioprotective or antihypertensive dietary advice,8 although both have been associated with lower incidences of cardiovascular events.9-11 A recent cross-sectional study suggests considerable hypotensive and cardioprotective effects of cocoa.12 Observational studies of the association between consumption of black or green tea and blood pressure yielded mixed results; some have reported a reduction of blood pressure,13-15 while others found no effects.16-18 These discrepancies may be due to potential biases and confounding factors that are in particular inherent to epidemiological studies of diet and disease.19 Several randomized controlled trials have also been conduced to answer the question of a causal relationship of cocoa20-24 and tea25-29 consumption on blood pressure, principally providing higher strength of evidence for an association with a dietary effect.30 We therefore undertook a prospective meta-analysis of randomized controlled trials to quantitatively assess the effect of cocoa or tea intake on blood pressure.
To identify randomized controlled studies that report the effects of cocoa or tea intake on blood pressure, we searched the electronic databases MEDLINE, EMBASE, SCOPUS, and Science Citation Index from 1966 to October 2006 as well as the Cochrane Controlled Trials Register for the medical subject headings (MeSH) and text words “cocoa,” “chocolate,” “tea,” “blood pressure,” “hypertension,” “endothelium,” and “cardiovascular.” We also compiled citations from the reference lists of original and review articles. Of the citations identified by the search terms (cocoa) OR (chocolate)/respectively (tea) AND (randomized controlled trial[Publication Type]) OR (randomized[Title/Abstract] AND controlled[Title/Abstract] AND trial[Title/Abstract]), the full articles were retrieved.
We considered studies in any language that were published as full articles. For inclusion, studies had to fulfill the following criteria: have a randomized controlled parallel group or crossover design; have examined at least 10 normotensive or hypertensive adults (age ≥18 years); report means (or differences between means) and standard deviations or 95% confidence intervals (CIs) of systolic blood pressure (SBP) and diastolic blood pressure (DBP) at baseline and after the intervention; and provide type, duration, and amount of the cocoa or tea consumption. Studies were excluded if only abstracts were published; information on cocoa or tea and control interventions was incomplete; allocation of participants to the treatments was not randomized; only supplements of tea or cocoa ingredients were used; or vitamin supplements or polyphenol-rich foods were concomitantly ingested or cocoa and tea intake was mixed with other dietary treatments. Data of multiple published reports from the same study population were included only once. Furthermore, studies with a duration of less than 7 days were excluded from the analysis. This cutoff value was set because shorter assessments (often only administrations of a single dose of cocoa or tea) were considered of questionable clinical relevance, and none of these very short-term studies we retrieved by our search strategy (Figure 1) reported changes in blood pressure after ingestion of cocoa or black and green tea.
Data extraction and quality assessment
Data were extracted independently by 2 investigators (D.T. and R.R.) with an interrater agreement31 value of κ = 0.94, and disagreements were resolved by consensus. Methodological quality of the selected studies was assessed independently by 2 reviewers (D.T. and R.R.) (κ = 0.89), and discrepancies were resolved by consensus. Randomized controlled trials were evaluated using the validated Jadad 11-item instrument with a maximum possible score of 13 points.32 Study quality was considered to be good when the score was greater than 9 points and poor when the score was 9 points or lower.
Extracted data include the first author's name; year of publication; country of investigation; number, age, sex, and health status of participants; losses to follow-up; concomitant medications; trial design and duration; Jadad score; funding sources; intervention assessment; and assessment of change in mean ± SD SBP and DBP.
Data synthesis and analysis
Changes in SBP and DBP in cocoa or tea and control groups are reported as differences between arithmetic means before and after intervention. If not reported, standard deviations of these differences were estimated by the following equation:
SDdifference = (SD2cocoa/tea + SD2control −[2 × R × SDcocoa/tea ×SDcontrol])1/2.
For the 2 studies in which subjects' individual pretreatment and posttreatment blood pressure values were available,20,23 we calculated values of the correlation coefficient R of greater than 0.85 for SBP and greater than 0.90 for DBP. To be conservative, we used an imputed value R of 0.68 according to the suggestions of the Cochrane Handbook for Systematic Reviews of Interventions. Crossover trials were incorporated in the meta-analysis as paired analyses if individual data were available. Otherwise, measurements from cocoa or tea and control intervention periods were considered in the same way as parallel group trials of cocoa or tea vs control by imputing the change estimates of the standard deviations.
Interstudy heterogeneity was assessed by the Cochrane Q test; P<.10 was considered statistically significant. The magnitude of heterogeneity was evaluated by the I2 statistic that describes the proportion of total variation in study estimates that is due to heterogeneity.33
To account for interstudy heterogeneity, the pooled estimates of the mean differences in SBP and DBP between control and intervention and the corresponding 95% CIs were calculated by the random effects model according to DerSimonian and Laird.34
Potential publication bias in the meta-analyses was assessed by the funnel plots of each trial's effect size against the inverse standard error. Funnel plot asymmetry was evaluated by the Egger regression test requiring a minimum of 5 trials to reliably detect a bias (P<.10).35 Adjusted estimates of the pooled changes in blood pressure and the overall 95% CIs were calculated by the trim-and-fill method according to Duval and Tweedie.36
To test whether any one study was exerting excessive influence on the results, we conducted a sensitivity analysis by systematically excluding each study and then reanalyzing the remaining data. Additional sensitivity analyses were done to test the influence of alternative values (0 and 1) of the imputed correlation coefficient R on the pooled estimates.
The statistical analyses were performed with Cochrane Review Manager 4.2 (Cochrane Library Software, Oxford, England) and MIX version 1.4 software (Department of Medical Informatics, Kitasato University, Kanagawa, Japan).
We identified 10 studies that met the inclusion criteria, with 5 addressing the relation between cocoa (Table 1) and 5 the relation between tea (Table 2) intake and blood pressure. Most studies were excluded because of short duration (<7 days) or missing information of randomization, withdrawals, or outcome (Figure 1). Two studies were excluded because supplements of cocoa or tea extracts were applied.37,38 One study26 assessed black tea and green tea in the same subjects in subsequent interventions. Because of the lack of independency between these studies and since black tea and green tea did not differ in their effects on blood pressure, we entered only the data of the black tea intervention. The cocoa studies had a combined total of 173 individuals allocated to cocoa (n = 87) and control (n = 86) arms, and the tea studies had a combined total of 343 individuals allocated to tea (n = 171) and control (n = 172) arms. The median duration of the interventions in the cocoa studies was 2 weeks, and in the tea studies, 4 weeks. Of the cocoa and tea study participants, 63.9% and 70.7%, respectively, were men and 34.0% and 48.8%, respectively, had hypertension or high-normal blood pressure.
Of 5 cocoa studies, 4 reported a reduction of SBP and DBP after cocoa consumption. Compared with the cocoa-free control, the pooled decrease was −4.7 mm Hg (95% CI, −7.6 to −1.8 mm Hg; P = .002) in SBP and −2.8 mm Hg (95% CI, −4.8 to −0.8 mm Hg; P = .006) in DBP for cocoa intake (Figure 2). Of the 5 studies on tea consumption, none was associated with significant alterations in blood pressure. Compared with control, the pooled change was 0.4 mm Hg (95% CI, −1.3 to 2.2 mm Hg; P = .63) in SBP and −0.6 mm Hg (95% CI, −1.5 to 0.4 mm Hg; P = .38) in DBP for tea intake (Figure 3).
There was evidence of considerable heterogeneity between the cocoa studies with respect to SBP (Q4 = 32.33; P<.001; I2 = 87.6%) as well as DBP (Q4 = 32.18; P<.001; I2 = 87.6%). In contrast, there was no indication of heterogeneity between the tea studies (SBP: Q4 = 0.61; P = .96; I2 = 0%; and DBP: Q4 = 3.29; P = .51; I2 = 0%). Exclusion sensitivity analysis showed that heterogeneity was due to the studies by Engler et al21 and Grassi et al.23 Omitting these studies had little impact on the pooled estimates for changes in SBP (−4.5 mm Hg [95% CI, −5.5 to −3.5 mm Hg]; P<.001) and DBP (−3.1 mm Hg [95% CI, −3.8 to −2.3 mm Hg]; P<.001).
Additional sensitivity analysis demonstrated that the values for the pooled changes in blood pressure with corresponding CIs and P values were not altered with the exclusion of any individual study or the imputation of other values of R.
The funnel plots and the Egger regression test suggested no significant asymmetry in the 4 meta-analyses (Figure 4). Furthermore, the trim-and-fill computation using the symmetry estimator L0 revealed that there were no missing trials, indicating that no publication bias was present.
The methodological quality score (Jadad scale) of cocoa and tea studies ranged from 8 to 10 (Table 1 and Table 2), with a mean (SD) of 9.2 (0.8) and 9.4 (0.9), respectively. With the exception of 1 study,21 participants were not reported to be blinded to the intervention. However, this problem is inherent to most dietary interventions. Further methodical deficiencies included failures to describe the methods to generate the sequence of randomization or to assess adverse effects and missing justification of the sample size. We found no indication that industrial or institutional funding affected the study outcomes with respect to blood pressure (Table 1 and Table 2). The concurrent administration of antihypertensive drugs along with black tea in the investigation by Duffy et al27 may have offset any antihypertensive effect of the tea; however, blood pressure–lowering effects of polyphenols have also been observed in normotensive subjects.
In the 4 cocoa studies, which were associated with blood pressure reductions, similar amounts of cocoa were applied to different study populations. The results suggest that younger subjects with mild essential hypertension experience the highest decrease in SBP and DBP, whereas elderly hypertensive subjects and younger normotensive subjects show smaller reductions (Table 1). Moreover, it appears that the amount of the ingested cocoa phenols is essential for the magnitude of the blood pressure reduction, since in the study by Engler et al,21 administration of about half of the cocoa phenols over the same 2-week period did not affect blood pressure.
The negative outcome of the tea interventions was independent of subjects' age, the presence of hypertension, or study duration (between 1-8 weeks). Furthermore, the reported cocoa and tea studies provided no indication that ethnicity, sex, or body weight affected outcome.
In our meta-analysis of randomized controlled trials in adults, diets rich in cocoa were associated with statistically significant reductions in SBP and DBP, whereas black or green tea did not lead to apparent changes in blood pressure. The magnitude of the hypotensive effects of cocoa is clinically noteworthy; it is in the range that is usually achieved with monotherapy of β-blockers or angiotensin-converting enzyme inhibitors.39 At the population level, a reduction of 4 to 5 mm Hg in SBP and 2 to 3 mm Hg in DBP would be expected to substantially reduce the risk of stroke (by about 20%), coronary heart disease (by 10%), and all-cause mortality (by 8%).40
The blood pressure–lowering effects of cocoa have a biological basis. Cocoa is a rich source of polyphenols.41 In mechanistic studies, cocoa extracts have been shown to cause arterial vasodilation by increasing endothelial production of nitric oxide.42 In clinical studies in healthy subjects, infusion of the nitric oxide synthase inhibitor NG-nitro-L-arginine methyl ester (L-NAME) caused doubling of SBP and DBP responses after only 4 days of ingestion of cocoa.43 These studies suggest that the polyphenols in cocoa-containing foods are likely to be responsible for the reduction in blood pressure and also the improvement of endothelial function44 and platelet inhibition45 by inducing local synthesis of the vasodilatory signaling molecule nitric oxide.
The lack of effects of tea on blood pressure appears less plausible. Tea is also rich in polyphenols,46 and the total polyphenol doses that were ingested with the tea diets were not lower compared with the cocoa diets (Table 1 and Table 2). Moreover, tea and cocoa studies showed no major differences in baseline characteristics of the participants or study duration. It is also unlikely that the dilator responses of the tea polyphenols are outweighted by pressor effects of the tea caffeine, since administration of caffeine-matched control beverages had no sustained impact (ie, lasting more than 60 minutes after consumption) on blood pressure.25,26 However, the composition of the polyphenols differs between cocoa and tea. The main polyphenol monomers in black and green tea are flavan-3-ols (in particular epicatechin gallates) and gallic acid46,47; the main polymers are condensed catechins (in particular, thearubigins and theaflavins) that dominate in black tea.47,48 The flavan-3-ols epicatechin and catechin are also present in cocoa, but the main cocoa polyphenols are procyanidins.41,49 Whereas the flavanols or gallic acid were found to exhibit no or only modest vasodilatory or nitric oxide–stimulating effects in different experimental settings50-54 and there are no data on vascular effects of thearubigins and theaflavins, the fraction of oligomeric procyanidins demonstrated a strong vasodilation.52,53 Furthermore, in humans, bioavailability of phenolic compounds from cocoa has been reported not only for the monomeric flavanols but also for the procyanidin oligomers.55 This suggests that the different plant phenols must be differentiated with respect to their blood pressure–lowering potential and thus cardiovascular disease prevention, supposing that the tea phenols are less active than cocoa phenols. In support of this conclusion, results of a component-based epidemiological study have shown that dietary flavanol intake was not associated with the incidence of myocardial infarction and stroke,56 while other flavonoids were found to be protective.57,58
We pooled the data of black and green tea interventions in a single meta-analysis because the principal polyphenol components are almost identical in black and green tea, and, although the relation of these components may vary between black and green tea, total polyphenols are in a similar concentration range.47
The present study provides robust effect estimates. The prospective design of the meta-analysis minimizes selection and recall biases. Despite residual statistical heterogeneity between the cocoa studies, the adjustments made by sensitivity analyses revealed no significant changes in pooled outcome measures.
Our findings have several potential limitations. First, as with any meta-analysis the internal validity relies on the quality of the individual studies. Although all studies were randomized and described adverse events or withdrawals, the lack of blinding of participants or investigators to the intervention in most of the studies reviewed increased the risk of expectation bias.
Second, our meta-analysis involved only a few studies with small sample sizes, which makes the estimates especially susceptible to publication bias and to overestimation of treatment effects; consequently, accuracy and statistical power of the outcome estimates were limited.59 Although the Egger regression test and trim-and-fill computation provided no indication of publication bias, this cannot be ruled out because these tests lacked sensitivity, with the inclusion of only 5 studies in our meta-analysis.
Third, the studies reviewed had only a short duration. Thus, their results cannot simply be translated into long-term outcomes, that is, the prediction of beneficial treatment effects. In particular, it has to be considered that the short-term administration and the calorie-balanced study design prevented a potential weight gain with the high-caloric cocoa diets (Table 1); however, a concurrent increase in body weight may reverse any blood pressure reductions during long-term habitual intake of cocoa products.60 Although outcome evidence from long-term randomized trials is ideal, those studies with dietary interventions are difficult to implement on a practical basis. It is therefore instructive to compare the data of our meta-analysis with the results of long-term observational studies.
A recent cross-sectional study that assessed habitual cocoa intake and blood pressure in 470 elderly men over 5 years found a −3.7 mm Hg (95% CI, −7.1 to −0.3 mm Hg) lower mean SBP and a −2.1 mm Hg (95% CI, −4.0 to −0.2 mm Hg) lower mean DBP in the highest tertile of cocoa intake compared with the lowest tertile.12 This is close to the pooled estimates we derived from the randomized trials but was observed with one tenth of the daily cocoa amount compared with the intake in the randomized trials. Hence, the long-term effects of high cocoa consumption on blood pressure may be underestimated by the presented meta-analysis of short-term trials. Moreover, the high degree of risk reduction of about 50% in cardiovascular and all-cause mortality associated with regular cocoa intake12 suggests that cocoa phenols also confer genuine cardiovascular protection beyond blood pressure reduction, possibly due to protective nitric oxide–mediated effects on endothelium or platelets.44,45
In contrast, long-term epidemiological studies of tea intake and blood pressure reported either no blood pressure–lowering effects of habitual tea consumption16-18 or only small reductions of approximately −2 mm Hg in SBP and −1 mm Hg in DBP,13-15 which is consistent with the nonsignificant effects in the short-term randomized trials. Accordingly, a meta-analysis of observational studies on tea consumption in relation to cardiovascular disease conducted in 2001 found only a small, nonsignificant reduction of myocardial infarction incidence with an increase in tea consumption of 3 cups per day (relative risk, 0.89; 95% CI, 0.79 to 1.01).9 Subsequent population studies also reported no61 or similar modest inverse associations62 of regular tea intake and cardiovascular diseases.
In conclusion, controlled data from short-term randomized and long-term observational studies suggest clinically relevant reductions of SBP and DBP with the use of cocoa products, supported by the biological plausibility and consistent laboratory data of the vasodilator activity of cocoa phenols. In contrast, cumulative evidence does not support substantial effects of tea consumption on blood pressure. The findings of favorable hypotensive cocoa actions should, however, not encourage common recommendations to consume more cocoa. We believe that any dietary advice must account for the high sugar, fat, and calorie intake with most cocoa products. On the basis of the limitations of current dietary studies, it appears reasonable to allow phenol-rich cocoa products such as dark chocolate for calorie-balanced substitution of high-fat dairy products, sugar confectionary, or cookies of the usual diet. Rationally applied, cocoa products might be considered part of dietary approaches to lower hypertension risk.
Correspondence: Dirk Taubert, MD, PhD, Department of Pharmacology, University Hospital of Cologne, Gleueler Str 24, D-50931 Cologne, Germany (firstname.lastname@example.org).
Accepted for Publication: December 18, 2006.
Author Contributions: Dr Taubert had full access to all of the data in the study and takes responsibility for the integrity of the data and the accuracy of the data analysis. Study concept and design: Taubert. Acquisition of data: Taubert and Roesen. Analysis and interpretation of data: Taubert, Roesen, and Schömig. Drafting of the manuscript: Taubert. Critical revision of the manuscript for important intellectual content: Taubert, Roesen, and Schömig. Statistical analysis: Taubert. Administrative, technical, and material support: Schömig. Study supervision: Taubert.
Financial Disclosure: None reported.
et al. National Heart, Lung, and Blood Institute Joint National Committee on Prevention, Detection, Evaluation, and Treatment of High Blood Pressure; National High Blood Pressure Education Program Coordinating Committee, The Seventh Report of the Joint National Committee on Prevention, Detection, Evaluation, and Treatment of High Blood Pressure: the JNC 7 report. JAMA
2003;2892560- 2572PubMedGoogle ScholarCrossref
European Society of Hypertension-European Society of Cardiology Guidelines Committee, 2003 European Society of Hypertension-European Society of Cardiology guidelines for the management of arterial hypertension. J Hypertens
2003;211011- 1053PubMedGoogle ScholarCrossref
HA The relation between dietary flavonol intake and coronary heart disease mortality: a meta-analysis of prospective cohort studies. Eur J Clin Nutr
2003;57904- 908PubMedGoogle ScholarCrossref
et al. The effect of fruit and vegetable intake on risk for coronary heart disease. Ann Intern Med
2001;1341106- 1114PubMedGoogle ScholarCrossref
GA Fruit and vegetable consumption and stroke: meta-analysis of cohort studies. Lancet
2006;367320- 326PubMedGoogle ScholarCrossref
JH Lifestyle, health and disease prevention: the underlying mechanisms. Eur J Cancer Prev
S1- S7PubMedGoogle Scholar
et al. Diet and lifestyle recommendations revision 2006: a scientific statement from the American Heart Association Nutrition Committee. Circulation
2006;11482- 96PubMedGoogle ScholarCrossref
CL Cocoa and chocolate flavonoids: implications for cardiovascular health. J Am Diet Assoc
2003;103215- 223PubMedGoogle ScholarCrossref
CL Evidence that the antioxidant flavonoids in tea and cocoa are beneficial for cardiovascular health. Curr Opin Lipidol
2002;1341- 49PubMedGoogle ScholarCrossref
D Cocoa intake, blood pressure, and cardiovascular mortality: the Zutphen Elderly Study. Arch Intern Med
2006;166411- 417PubMedGoogle Scholar
OP Tea consumption: relationship to cholesterol, blood pressure, and coronary and total mortality. Prev Med
1992;21546- 553PubMedGoogle ScholarCrossref
RL Tea intake is inversely related to blood pressure in older women. J Nutr
2003;1332883- 2886PubMedGoogle Scholar
CJ The protective effect of habitual tea consumption on hypertension. Arch Intern Med
2004;1641534- 1540PubMedGoogle ScholarCrossref
MA The relationships between alcoholic beverage use and other traits to blood pressure: a new Kaiser Permanente study. Circulation
1986;73628- 636PubMedGoogle ScholarCrossref
et al. Habitual coffee consumption and blood pressure: a study of self-defense officials in Japan. Eur J Epidemiol
1998;14669- 673PubMedGoogle ScholarCrossref
KM Evaluating epidemiologic evidence of the effects of food and nutrient exposures. Am J Clin Nutr
1999;691339S- 1344SPubMedGoogle Scholar
W Chocolate and blood pressure in elderly individuals with isolated systolic hypertension. JAMA
2003;2901029- 1030PubMedGoogle ScholarCrossref
et al. Flavonoid-rich dark chocolate improves endothelial function and increases plasma epicatechin concentrations in healthy adults. J Am Coll Nutr
2004;23197- 204PubMedGoogle ScholarCrossref
C Short-term administration of dark chocolate is followed by a significant increase in insulin sensitivity and a decrease in blood pressure in healthy persons. Am J Clin Nutr
2005;81611- 614PubMedGoogle Scholar
et al. Cocoa reduces blood pressure and insulin resistance and improves endothelium-dependent vasodilation in hypertensives. Hypertension
2005;46398- 405PubMedGoogle ScholarCrossref
et al. Regular consumption of a flavanol-rich chocolate can improve oxidant stress in young soccer players. Clin Dev Immunol
2005;1211- 17PubMedGoogle ScholarCrossref
et al. Effect of black tea drinking on blood lipids, blood pressure and aspects of bowel habit. Br J Nutr
1997;7841- 55PubMedGoogle ScholarCrossref
N Effects on blood pressure of drinking green and black tea. J Hypertens
1999;17457- 463PubMedGoogle ScholarCrossref
et al. Short- and long-term black tea consumption reverses endothelial dysfunction in patients with coronary artery disease. Circulation
2001;104151- 156PubMedGoogle ScholarCrossref
IB Regular ingestion of tea does not inhibit in vivo lipid peroxidation in humans. J Nutr
2002;13255- 58PubMedGoogle Scholar
H Randomized controlled trial for an effect of green tea consumption on insulin resistance and inflammation markers. J Nutr Sci Vitaminol (Tokyo)
2005;51335- 342PubMedGoogle ScholarCrossref
US Food and Drug Administration, Center of Food Safety and Applied Nutrition, Office of Special Nutritionals, Guidance for Industry: Significant Scientific Agreement in the Review of Health Claims for Conventional Foods and Dietary Supplements. Washington, DC US Food and Drug Administration1999;
GG Tips for learners of evidence-based medicine, 3: measures of observer variability (kappa statistic). CMAJ
2004;1711369- 1373PubMedGoogle ScholarCrossref
et al. Assessing the quality of reports of randomized clinical trials: is blinding necessary? Control Clin Trials
1996;171- 12PubMedGoogle ScholarCrossref
R Trim and fill: a simple funnel-plot-based method of testing and adjusting for publication bias in meta-analysis. Biometrics
2000;56455- 463PubMedGoogle ScholarCrossref
et al. Dietary flavanols and procyanidin oligomers from cocoa (Theobroma cacao
) inhibit platelet function. Am J Clin Nutr
2003;771466- 1473PubMedGoogle Scholar
MS Metabolic effects of green tea and of phases of weight loss. Physiol Behav
2006;87185- 191PubMedGoogle ScholarCrossref
RJ ACE inhibitors, beta-blockers, calcium blockers, and diuretics for the control of systolic hypertension. Am J Hypertens
2001;14241- 247PubMedGoogle ScholarCrossref
CY Cocoa has more phenolic phytochemicals and a higher antioxidant capacity than teas and red wine. J Agric Food Chem
2003;517292- 7295PubMedGoogle ScholarCrossref
CT Effects of cocoa extracts on endothelium-dependent relaxation. J Nutr
2000;1302105S- 2108SPubMedGoogle Scholar
NK Flavanol-rich cocoa induces nitric-oxide-dependent vasodilation in healthy humans. J Hypertens
2003;212281- 2286PubMedGoogle ScholarCrossref
Nutrient Data Laboratory, Food Composition Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, US Department of Agriculture, USDA Database for the Flavonoid Content of Dried Teas: Release 2. Beltsville, Md Nutrient Data Laboratory2006;
LVan De Put
CA Black tea represents a major source of dietary phenolics among regular tea drinkers. Free Radic Res
2002;361127- 1135PubMedGoogle ScholarCrossref
et al. Analyses of polyphenols in cacao liquor, cocoa, and chocolate by normal-phase and reversed-phase HPLC. Biosci Biotechnol Biochem
2000;642581- 2587PubMedGoogle ScholarCrossref
R Nitric oxide production and endothelium-dependent vasorelaxation induced by wine polyphenols in rat aorta. Br J Pharmacol
1997;1201053- 1058PubMedGoogle ScholarCrossref
D Vasorelaxation, endothelium, and wine. Biofactors
1997;6455- 459Google ScholarCrossref
RM Isolation and characterization of endothelium-dependent vasorelaxing compounds from grape seeds. J Agric Food Chem
2000;486384- 6390PubMedGoogle ScholarCrossref
R Nitric oxide formation and corresponding relaxation of porcine coronary arteries induced by plant phenols: essential structural features. J Cardiovasc Pharmacol
2002;40701- 713PubMedGoogle ScholarCrossref
JL Vasorelaxant effects of grape polyphenols in rat isolated aorta: possible involvement of a purinergic pathway. Fundam Clin Pharmacol
2003;17673- 681PubMedGoogle ScholarCrossref
et al. Procyanidin dimer B2 [epicatechin-(4beta-8)-epicatechin] in human plasma after the consumption of a flavanol-rich cocoa. Am J Clin Nutr
2002;76798- 804PubMedGoogle Scholar
EJBueno de Mesquita
D Catechin intake might explain the inverse relation between tea consumption and ischemic heart disease: the Zutphen Elderly Study. Am J Clin Nutr
2001;74227- 232PubMedGoogle Scholar
D Dietary flavonoids, antioxidant vitamins, and incidence of stroke: the Zutphen study. Arch Intern Med
1996;156637- 642PubMedGoogle ScholarCrossref
PC Polyphenols and disease risk in epidemiologic studies. Am J Clin Nutr
317S- 325SPubMedGoogle Scholar
M Obesity and hypertension: epidemiological and clinical issues. Eur Heart J
57- 70PubMedGoogle ScholarCrossref
IM Lack of association between tea and cardiovascular disease in college alumni. Int J Epidemiol
2003;32527- 533PubMedGoogle ScholarCrossref
et al. Green tea consumption and mortality due to cardiovascular disease, cancer, and all causes in Japan: the Ohsaki study. JAMA
2006;2961255- 1265PubMedGoogle ScholarCrossref