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Article
January 1940

EFFECT OF THE RETICULOCYTOGENIC PRINCIPLE IN URINE IN THE TREATMENT OF PERNICIOUS ANEMIA

Author Affiliations

CHICAGO

From the Department of Physiology, College of Medicine, University of Illinois, and the Department of Physiology and Pharmacology, University of Louisville School of Medicine.

Arch Intern Med (Chic). 1940;65(1):21-25. doi:10.1001/archinte.1940.00190070031002
Abstract

Normal human urine contains a substance which, like the antipernicious anemia principle in liver, is reticulocytogenic for the pigeon, rat and guinea pig.1 An extract of normal human urine prepared by a method basically that used for liver extracts has been found to be reticulocytogenic for the pigeon.2 Decastello3 reported that normal human urine administered intramuscularly or rectally is effective in the treatment of pernicious anemia, and McCann4 found that kidney given by mouth is effective. These findings suggested the possible identity or similarity of the reticulocytogenic principle in urine and the hemopoietic principle in liver. In view of the nonspecificity of the pigeon, rat and guinea pig bioassay methods for the hemopoietic principle in liver, the possibility of urinary excretion of the principle was studied by administering the urine extract previously mentioned orally and intramuscularly to patients with pernicious anemia. A preliminary report of the effect of intramuscular injections of

References
1.
Wakerlin, G. E., and Bruner, H. D.:  Presence in Normal Human Urine of a Reticulocyte-Stimulating Principle for the Pigeon ,  Arch. Int. Med. 57:1032 ( (May) ) 1936.Crossref
2.
Liener, G.:  Ueber antianämisch wirksame Substanzen im Harn ,  Wien. klin. Wchnschr. 48:559, 1935.
3.
Walters, O. S.:  Reticulocytogenic Action of Pernicious Anemia Urine Extracts ,  Am. J. Physiol. 116:158, 1936.
4.
Wakerlin, G. E.; Bruner, H. D., and Kinsman, J. M.:  A Modified Pigeon Method for the Bioassay of Anti-Pernicious Anemia Liver Extracts ,  J. Pharmacol. & Exper. Therap. 58:1, 1936.
5.
Decastello, A.:  Ueber antianämisch wirksame Substanzen im Harn ,  Med. Klin. 31:377, 1935.
6.
McCann, W. S.:  Effect of Kidney on Blood Regeneration in Pernicious Anemia ,  Proc. Soc. Exper. Biol. & Med. 25:255, 1928.
7.
Wakerlin, G. E.:  The Relation of the Reticulocytogenic Urine Principle to the Hemopoietic or Antipernicious Anemia Liver Principle ,  Am. J. Physiol. 119:417, 1937.
8.
Wakerlin, G. E.:  Presence of Anti-Pernicious Anemia Principle in Normal Human Urine ,  Proc. Soc. Exper. Biol. & Med. 32:1607, 1935.
9.
Jequier, E., and Apsey, G. R. M.:  The Anti-Pernicious Principle: Some Experiments with Urine ,  Brit. M. J. 2:934, 1938.
10.
Wakerlin, G. E.: Unpublished data.
11.
Jequier and Apsey.7
12.
The kidney extracts were supplied by the Lederle Laboratories, Inc., Pearl River, N. Y. (Dr. Guy W. Clark), and the Abbott Laboratories, North Chicago (Dr. J. F. Biehn).
13.
Dakin, H. D.; Ungley, C. C., and West, R.:  Further Observations on the Chemical Nature of a Hematopoietic Substance Occurring in Liver ,  J. Biol. Chem. 115:771, 1936.
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