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March 1960

Pathogenic Staphylococci Isolated at Boston City Hospital in 1958: Phage Types and Antibiotic Susceptibility

Author Affiliations


From the Thorndike Memorial Laboratory, Second and Fourth (Harvard) Medical Services, Boston City Hospital, and the Department of Medicine, Harvard Medical School, Boston. Present address of Dr. Hirsch: Munich, Germany; Dr. Wallmark is a Leaderle International Fellow, on leave from the State Bacteriological Laboratory, Stockholm, Sweden.

AMA Arch Intern Med. 1960;105(3):383-397. doi:10.1001/archinte.1960.00270150037005

In a recent paper, Finland, Jones, and Bennett1 reported on the antibiotic sensitivity and phage types of pathogenic staphylococci isolated from hospitalized patients and autopsies at the Boston City Hospital during the latter half of 1955. Some relationship was demonstrated between resistance of strains to the most widely used antibiotics and prior antibiotic therapy of the patients from whom the strains were obtained. It was also shown that marked differences in the antibiotic-resistant pattern of strains isolated at different times from the same source or from different sources at the same time in any given patient were usually related to the occurrence or introduction of different strains, as judged by their phage types, and only occasionally were they related directly to the antibiotic or antibiotics previously used in the treatment of the patient.

The present study was carried out on strains of Staphylococcus aureus isolated from infected patients at

Marion E. Lamb and A. Kathleen Daly of the Bacteriology Laboratory, Mallory Institute of Pathology isolated most of these strains.
Carried out with the assistance of Clare Wilcox and Ann Najarian.
Carried out with the assistance of Mildred W. Barnes.
Supplied, with their propagating strains, by Dr. John E. Blair, Hospital for Joint Diseases, New York.
Thiocymetin has been given the generic name dextrosulphenidol and was originally designated Win 5063-2; U 15,442 is the same as Win 5094-2. These drugs were supplied to us by Upjohn Company which obtained them from Winthrop Laboratories.
The nontypable strains were sent to Dr. John E. Blair and were retyped in his laboratory. None reacted with any phages at RTD, but 10 showed lysis with 1,000 X RTD of 1 or more types, mostly of Groups I and III, half of them only weakly. Only one reacted with 80/81 phages.
Finland, M.; Jones, Jr., W. F., and Bennett, I. L., Jr.:  Antibiotic Susceptibility and Phage Types of Pathogenic Staphylococci: A Study of 210 Strains Isolated at Boston City Hospital in 1955 ,  A.M.A. Arch. Int. Med. 104:365-377 ( (Sept.) ) 1959.Crossref
Bynoe, E. T.; Elder, R. H., and Comtois, R. D.:  Phage-Typing and Antibiotic-Resistance of Staphylococci Isolated in a General Hospital ,  Canad. J. Microbiol. 2:346-358 ( (May) ) 1956.Crossref
Wallmark, G.:  Bacteriophage Typing of Staphylococcus Aureus Pyogenes: I. A Report of the Method and Some Experimental Results ,  Acta path. et microbiol. scandinav. 34:57-67, 1954.Crossref
Haskell, T. H.; French, J. C., and Bartz, Q. R.:  Paromomycin ,  J. Am. Chem. Soc. 81:3480-3483 ( (July) ) 1959.Crossref
Haight, T. H., and Finland, M.:  Modified Gots Test for Penicillinase Production ,  Am. J. Clin. Path. 22:806-808 ( (Aug.) ) 1952.
Kunin, C. M., and Finland, M.:  Absorption and Urinary Excretion of Chloramphenicol and 2 Analogues in Normal Man ,  Proc. Soc. Exper. Biol. & Med. , to be published.
Blair, J. E., and Bynoe, E. T.:  Variation in 3 Staphylococcal Typing Phages ,  Pub. Health Rep. 73:465-466 ( (May) ) 1958.Crossref