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March 1960

Relationship of Viruses to Malignant Disease: Part II. Oncogenic Properties of Cell-Free Filtrates of Human Tumors

Author Affiliations
Roswell Park Memorial Institute, Buffalo.
AMA Arch Intern Med. 1960;105(3):482-491. doi:10.1001/archinte.1960.00270150136013

The fact that viruses can cause cancer in a wide variety of animal species is now firmly established 1-8; and the list of known animal viral tumor agents is steadily growing. Since basic biological phenomena are generally quite similar throughout the animal kingdom, it would be surprising indeed if humans were not susceptible to viral oncogenesis. Barriers encountered in investigations of animal-tumor viruses point up the exceedingly difficult problems one might expect to encounter in a search for human-tumor viruses. On the other hand, study of these agents has supplied much valuable information concerning the complex inter-play of other biological factors which influence the occurrence of virus-induced tumors. Much has been learned concerning the importance of genetic and hormonal factors. The importance of genetic factors has been best evaluated in mice because of the large number of highly inbred strains available. For example, the ability of the Bittner mammary-tumor agent

Obtained from Microbiological Associates, Bethesda, Md.
Clark, R. L., Jr. (Editor):  Introduction to Viruses and Tumor Growth, Eleventh Annual Symposium on Fundamental Cancer Research ,  Texas Rep. Biol. & Med. 15:1-378, 1957.
Furth, J., and Metcalf, D.:  An Appraisal of the Tumor-Virus Problem ,  J. Chron. Dis. 8:88, 1958.
Oberling, C., and Guerin, M.:  The Role of Viruses in the Production of Cancer ,  Advances in Cancer Research 2:253, 1954.
Stanley, W. M.:  Relationships, Established and Prospective, Between Viruses and Cancer ,  Ann. New York Acad. Sc. 71:1100, 1958.
Whitelock, O. S., Editor:  Subcellular Particles in the Neoplastic Process ,  Ann. New York Acad. Sc. 68:245-656, 1957.
Beard, J. W.; Sharp, D. G., and Eckert, E. A.:  Tumor Viruses ,  Advances in Virus Research 3:149, 1955.
Burnet, M.:  Cancer, A Biological Approach: I. The Processes of Control ,  Brit. M.J. 1:779, 1957.
Duran-Reynals, F.:  Virus-Induced Tumors and the Virus Theory , in  Pathophysiology of Cancer , Homburger, F., and Fishman, W. H., Editors, New York, Paul B. Hoeber, Inc. (Medical Book Department of Harper & Brothers), 1953, p. 298.
Bittner, J. J.:  Bertner Foundation Lecture: Studies on Mammary Cancer in Mice and Their Implications for the Human Problem ,  Tex. Rep. Biol. & Med. 15:659, 1957.
Andervont, H. B., and Dunn, T. B.:  Influence of Heredity and the Mammary Tumor Agent on the Occurrence of Mammary Tumors in Hybrid Mice ,  J. Nat. Cancer Inst. 14:317, 1953.
Gross, L.:  "Spontaneous" Leukemia Developing in C3H Mice Following Inoculation in Infancy with AK-Leukemic Extracts or AK-Embryos ,  Proc. Soc. Exper. Biol. & Med. 76:27, 1951.
Duran-Reynals, F., and Thomas, R. M.:  A Hemorrhagic Disease Occurring in Chicks Inoculated with the Rous and Fujinami Viruses ,  Yale J. Biol. & Med. 13:77, 1940.
Beard, J. W.:  Etiology of Avian Leukosis ,  Ann. New York Acad. Sc. 68:473, 1957.
Stewart, S. E.; Eddy, B. E.; Gochenour, A. M.; Borgese, N. G., and Grubbs, A. G.:  The Induction of Neoplasms with a Substance Released from Mouse Tumors by Tissue Culture ,  Virology 3:380, 1957.
Mirand, E. A.; Mount, D. T.; Moore, G. E.; Grace, J. T., Jr., and Sokal, J. E.:  Induction of Tumors by Virus-like Agent(s) Released by Tissue Culture ,  Proc. Soc. Exper. Biol. & Med. 99:1, 1958.
Rose, S. M., and Rose, F. C.:  Tumor Agent Transformations in Amphibia ,  Cancer Res. 12:1, 1952.
Burmester, B. R.:  Routes of Natural Infection in Avian Lymphomatosis ,  Ann. New York Acad. Sc. 68:487, 1957.
Mühlboch, O.:  Studies on the Transmission of the Mammary Tumor Agent by the Male Parent ,  J. Nat. Cancer Inst. 12:819, 1952.
Rowe, W. P.; Hartley, J. W.; Law, L. W., and Huebner, R. J.:  Studies of Mouse Polyoma Virus Infection: III. Distribution of Antibodies in Laboratory Mouse Colonies ,  J. Exper. Med. 109: 449, 1959.
Rowe, W. P.; Hartley, J. W.; Brodsky, I.; Huebner, R. J., and Law, L. W.:  Observations on the Spread of Mouse Polyoma Virus Infection ,  Nature, London 182:1617, 1958.
Andervont, H. B.:  Relation of Milk Influence to Mammary Tumors of Hybrid Mice ,  J. Nat. Cancer Inst. 5:391, 1946.
Waters, N. F.:  Breeding for Resistance and Susceptibility to Avian Lymphomatosis ,  Poultry Sc. 24:259, 1945.Crossref
Greenwood, A. W.; Blyth, J. S., and Carr, J. G.:  Indications of the Heritable Nature of Non-Susceptibility to Rous Sarcoma in Fowls ,  Brit. J. Cancer 2:135, 1948.Crossref
Andervont, H. B.: Biological Background for Experimental Work on Tumors, Canadian Cancer Conference, edited by R. W. Begg, New York, Academic Press, Inc., 1:1-24, 1955.
Andervont, H. B.:  Genetic, Hormonal and Age Factors in Susceptibility and Resistance to Tumor-Inducing Viruses ,  Texas Rep. Biol. & Med. 15:462, 1957.
Mirand, E. A.; Grace, J. T.; Moore, G. E., and Mount, D. T.:  Relationship of Viruses to Malignant Disease: I. Tumor Induction by SE Polyoma ,  Arch. Int. Med. 105:469 ( (Feb.) ) 1960.
Unpublished data.
Wile, U. J., and Kingery, L. B.:  The Etiology of Common Warts ,  J.A.M.A. 73:970, 1919.
Eddy, B. E.; Stewart, S. E.; Young, R., and Mider, G. B.:  Neoplasms in Hamsters Induced by Mouse Tumor Agent Passed in Tissue Culture ,  J. Nat. Cancer Inst. 20:747, 1958.
Andervont, H. B.:  Susceptibility of Young and Adult Mice to the Mammary-Tumor Agent ,  J. Nat. Cancer Inst. 5:397-401, 1945.
Gross, L.:  Development and Serial Cell-Free Passage of a Highly Potent Stain of Mouse Leukemia Virus ,  Proc. Soc. Exper. Biol. & Med. 94:767, 1957.
Graffi, A.:  Chloroleukemia of Mice ,  Ann. New York Acad. Sc. 68:540, 1957.
Friend, C. J.:  Cell-Free Transmission in Adult Swiss Mice of a Disease Having the Character of a Leukemia ,  J. Exper. Med. 105:307, 1957.
Schwartz, S. O.; Schoolman, H. M., and Szanto, P. B.:  The Enhancement of the Development of AKR Lymphoma by Cell-Free Filtrates of Brain ,  J. Lab. & Clin. Med. 46:949, 1955.
Woolley, G. W., and Small, M. C.:  Experiments on Cell-Free Transmission of Mouse Leukemia ,  Cancer 9:1102, 1956.
Franks, W. R.; McGregor, A.; Shaw, M. M., and Skubbis, J.:  Development of Leukosis by Cell-Free Filtrates of Solid Tumors or in Mice Surviving Immune to These Tumors ,  Proc. Am. A. Cancer Res. 3:19, 1959.
Moloney, J. B.:  Preliminary Studies on a Mouse Lymphatic Leukemia Virus Extracted from Sarcoma 37 ,  Proc. Am. A. Cancer Res. 3:44, 1959.
Lieberman, M., and Kaplan, H. S.:  Leukemogenic Activity of Filtrates from Radiation-Induced Lymphoid Tumors of Mice ,  Science 130:387, 1959.
Gross, L.:  Serial Cell-Free Passage of a Radiation-Activated Mouse Leukemia Agent ,  Proc. Soc. Exper. Biol. & Med. 100:102, 1959.
Schwartz, S. O.; Schoolman, H. M.; Szanto, P. B., and Spurrier, W.:  Studies in Leukemia: VI. The Induction of Leukemia in AKR Mice by Means of Cell-Free Filtrates of Humans Who Died of Leukemia ,  Cancer Res. 17:218, 1957.
Bergolt's, V. M.:  Experimental Studies of the Etiology of Leukemia in Man: I. The Discovery in Leukemic Tissues from Man of a Cell-Free Factor Producing Leukemia in Mice, Probl. Hemat. , (English edition: Problems of Hematology & Blood Transfusions)  Moscow 2:11, 1957.
Dmochowski, L.; Sykes, J. A.; Grey, C. E.; Shullenberger, C. C., and Howe, C. D.:  Studies on Human Leukemia ,  Proc. Am. A. Cancer Res. 3: 17, 1959.
Kilham, L.:  Metastasizing Viral Fibromas of Gray Squirrels: Pathogenesis and Mosquito Transmission ,  Am. J. Hyg. 61:55, 1955.
Kilham, L., and Dalmat, H. T.:  Host-Virus-Mosquito Relationship of Shope Fibromas in Cottontail Rabbits ,  Am. J. Hyg. 61:45, 1955.
Hauschka, T. S.: Personal communication to the authors.
Leuchtenberger, C.:  Cytoplasmic "Inclusion Bodies" Containing Desoxyribose Nucleic Acid (DNA) in Cells of Human Rectal Polyps ,  Lab. Invest. 3:132, 1954.