[Skip to Content]
[Skip to Content Landing]
Article
July 1973

Urinary ß-Glucuronidase Activity in Renal Disease

Author Affiliations

Los Angeles

From the departments of medicine and surgery (urology), UCLA School of Medicine, Los Angeles.

Arch Intern Med. 1973;132(1):63-69. doi:10.1001/archinte.1973.03650070055008
Abstract

Urinary β-glucuronidase activity was measured in dialyzed urine samples from normal subjects and patients with renal disease of diverse causes. The normal mean value was 14.4 enzyme units with a standard deviation of 7.1 and a range of 3.1 to 32.0 enzyme units. Urinary enzyme activity was increased in the majority of patients with active renal parenchymal disease, particularly those diseases resulting in tubular injury. Measurement of urinary β-glucuronidase activity was useful in detecting acute tubular necrosis, early renal transplant rejection, active pyelonephritis, and renal adenocarcinoma. It was not possible to differentiate upper from lower urinary tract infection.

References
1.
Wacker WEC, Dorfman LE:  Urinary lactic dehydrogenase activity: I. Screening method for detection of cancer of kidneys and bladder.  JAMA 181:972-978, 1962.Crossref
2.
Dorfman LE, Amador E, Wacker WEC:  Urinary lactic dehydrogenase activity: II. Elevated activities for diagnosis of carcinomas of kidney and bladder.  Biochem Clin 2:41, 1963.
3.
Dorfman LE, Amador E, Wacker WEC:  Urinary lactic dehydrogenase activity: III. An analytical validation of the assay method.  JAMA 184:1-6, 1963.Crossref
4.
Wacker WEC, Dorfman LE, Amador E:  Urinary lactic dehydrogenase activity: IV. Screening test for detection of renal disease dissociated and in association with arterial hypertension.  JAMA 188:671-676, 1964.Crossref
5.
Amador E, Zimmerman TS, Wacker WEC:  Urinary alkaline phosphatase activity: I. Elevated urinary LDH and alkaline phosphatase activities for the diagnosis of renal adenocarcinomas.  JAMA 185:769-775, 1963.Crossref
6.
Amador E, Zimmerman TS, Wacker WEC:  Urinary alkaline phosphatase activity: II. An analytical validation of the assay method.  JAMA 185:953-957, 1963.Crossref
7.
Amador E, Dorfman LE, Wacker WEC:  Urinary alkaline phosphatase and lactic dehydrogenase activity in the differential diagnosis of renal diseases.  Ann Intern Med 62:30-40, 1965.Crossref
8.
Riggins RS, Kiser WS:  A study of lactic dehydrogenase in urine and serum of patients with urinary tract disease.  J Urol 90:594-601, 1963.
9.
Butterworth PJ, et al:  Patterns of urinary excretion of alkaline phosphatase in acute renal failure.  Clin Chim Acta 11:212-219, 1965.Crossref
10.
Goldberg WM, Chakrabarti S, Filipich R:  Urinary lactic dehydrogenase, alkaline phosphatase and lysozyme studies in renal disease.  Can Med Assoc J 94:1264-1268, 1966.
11.
Rosalki SB, Wilkinson JH:  Preliminary communication—Urinary lactic dehydrogenase in renal disease.  Lancet 2:327-328, 1958.
12.
Crockson RA:  Lactic dehydrogenase in renal disease: Urinary concentration and relative clearances.  Lancet 1:140-142, 1961.Crossref
13.
Brenner BM, Gilbert VE:  Elevated levels of lactic dehydrogenase, glutamic-oxaloacetic transaminase and catalase in infected urine.  Am J Med Sci 245:31-42, 1963.Crossref
14.
Hayslett JP, Periuie PE, Finch SC:  Urinary muramidase and renal disease: Correlation with renal histology and implication for the mechanism of enzymuria.  N Engl J Med 279:506-512, 1968.Crossref
15.
Wachstein M:  Histochemical staining reactions of the normally functioning and abnormal kidney.  J Histochem Cytochem 3:246-270, 1955.Crossref
16.
Bank N, Bailine SH:  Urinary beta-glucuronidase activity in patients with urinary tract infection.  N Engl J Med 272:70-75, 1965.Crossref
17.
Paul W, Schapiro A, Gonick H:  Studies of human kidney and urine beta-glucuronidase: I. Biochemical characteristics.  Enzyme 8:47-66, 1967.
18.
Schapiro A, Paul W, Gonick H:  Studies of human kidney and urine beta-glucuronidase: II. In vitro and in vivo factors influencing measurement.  Enzyme 8:135-148, 1967.
19.
Abul-Fadl MAM:  Inhibition and activation of β-glucuronidase in urine.  Biochem J 65:16-17, 1957.
20.
Goldberg JA, et al:  A method for the colorimetric determination of beta-glucuronidase in urine, serum, and tissue: Assay of enzymatic activity in health and disease.  Gastroenterology 36:193-201, 1959.
21.
Lewis FJW, Plaice HJ:  Urinary beta-glucuronidase activity in cancer of the bladder and other diseases.  Br J Cancer 14:106-120, 1960.Crossref
22.
Conzelman GM Jr, Crout DW:  A non-competitive β-glucuronidase inhibitor in rabbit urine.  Proc Soc Exp Biol Med 107:372-374, 1961.Crossref
23.
Folin OKO, Wu H:  A system of blood analysis.  J Biol Chem 38:81-110, 1919.
24.
Roberts AP, et al:  Estimation of beta-glucuronidase activity in urinary-tract infection.  N Engl J Med 276:1468-1470, 1967.Crossref
25.
Drinkard JP, et al:  Azathioprine and prednisone in the treatment of adults with lupus nephritis: Clinical, histological, and immunological changes with therapy.  Medicine 49:411-432, 1970.Crossref
26.
Kallet HA, Lapco L:  Urine beta-glucuronidase activity in urinary tract disease.  J Urol 97:352-356, 1967.
27.
Hayashi M:  Distribution of beta-glucuronidase activity in rat tissues employing the naphthol AS-BI glucuronide hexazonium pararosanilin method.  J Histochem Cytochem 12:659-669, 1964.Crossref
28.
Fripp PJ:  The origin of urinary beta-glucuronidase.  Br J Cancer 19:330-335, 1965.Crossref
29.
Kerr WK, et al:  Biochemical factors in the etiology of carcinoma of the bladder: The role of β-glucuronidase.  Cancer 16:633-638, 1963.Crossref
30.
Boyland E, Wallace DM, Williams DC:  The activity of the enzymes sulphatase and beta-glucuronidase in the urine, serum and bladder tissue.  Br J Cancer 9:62-79, 1955.Crossref
31.
Ronald A, Cutler R, Turck M:  Parameters distinguishing "upper" from "lower" recurrent infections of the urinary tract, abstracted.  Clin Res 15:310, 1967.
32.
Schapiro A, Paul W, Gonick H:  Urinary beta-glucuronidase in urologic diseases of the kidneys.  J Urol 100:146-157, 1968.
33.
Coonrod D, Paterson PY:  Urine beta-glucuronidase in renal injury: I. Enzyme assay conditions and response to mercuric chloride in rats.  J Lab Clin Med 73:6-16, 1969.
34.
Porter KA:  Experience in Renal Transplantation . Philadelphia, WB Saunders Co, 1964, pp 299-359.
35.
Noble RE, Najarian JS, Brainerd HD:  Urine and serum lysozyme measurement in renal homo-transplantation.  Proc Soc Exp Biol Med 120:737-740, 1965.Crossref
36.
Musa BU, Doe RP, Seal US:  Purification and properties of human liver β-glucuronidase.  J Biol Chem 240:2811-2816, 1965.
37.
Melicow MM, Uson AC, Lipton R:  β-glucuronidase activity in the urine of patients with bladder cancer and other conditions.  J Urol 86:89-94, 1961.
×