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Article
May 1914

EXPERIMENTAL STUDIES ON THE BLOOD-SERUM OF COWS IMMUNIZED AGAINST TUBERCULOSIS: "SENSITIZATION" OF LIVING TUBERCLE BACILLI

Author Affiliations
SARANAC LAKE, N. Y.
From Saranac Laboratory for the Study of Tuberculosis, Dr. E. L. Trudeau, Director.
Arch Intern Med (Chic). 1914;XIII(5):682-700. doi:10.1001/archinte.1914.00070110011002
Abstract

The experiments given in detail in this contribution relate to some calves that were immunized in 1902 and 1903 by intravenous inoculations of human tubercle bacilli. The results of various serum reactions, including complement deviation, agglutination and precipitation, were published in 1904.1 Bactericidal and bacteriolytic tests, which were briefly described at that time,2 were also performed with culture bacilli.

Nearly all the calves were killed at the conclusion of the first-mentioned experiments. Calf 5 was, however, retained for more than ten years, being repeatedly inoculated with cultures and extracts of tubercle bacilli. These were always of the human type, so that the actual test of immunity-inoculation of bovine bacilli was never made. Nevertheless, from the well-known protection acquired by calves under similar treatment, it is fair to assume that this animal was at least relatively immune. The further study of immune serums in general, and of

References
1.
 Studies from the Saranac Laboratory ,  Jour. Med. Research , 1904, xii, 215.
2.
 Studies from the Saranac Laboratory ,  Jour. Med. Research , 1904, xii, 226.
3.
 Opsonins in Tuberculosis ,  Tr. Assn. Am. Phys. , 1907, p. 517.
4.
Neporoschny, S. D. (quoted by Pawlowsky) :  Ztschr. f. Tuberk. , 1911, xvii, 8.
5.
Ruppel, W. G., and Rickman, W.:  Ztschr. f. Immunitätsforsch. , 1910, vi, 344.
6.
Calmette, A., and Guérin, C.:  Ann. de l'Inst. Pasteur , 1911, xxv, 629.
7.
Pawlowsky, A. D.:  Ztschr. f. Tuberk. , 1911, xvii, 1.
8.
Sata, A.:  Ztschr. f. Tuberk. , 1911, xviii, 1
9.
1913, xx, 1.
10.
Meyer, F.:  Berl. klin. Wchnschr. , 1910, No. (20) , p. 926.
11.
Neporoschny, reviewed by Piel P.:  Beitr. z. Klin. d. Tuberk. , xxi, 311.
12.
With Koch's agglutination emulsion, diluted 1 to 1,000.
13.
The agglutination titer of Serum 15 never exceeded 1 to 100, nor was any precipitin obtained. The animal was slaughtered December, 1907, and the lungs and other viscera were normal in appearance. The lung-hilus lymph-nodes were also normal except that the largest showed a white cicatrix in its center. No inoculations were made with it so that its tuberculous source could only be surmised.
14.
In March, 1907, some experiments were undertaken with rabbits inoculated intravenously with virulent human tubercle bacilli treated with Serums 15 and 5, and that of Cow 16 ( normal ), for a control, together with broth and normal saline controls. After the inoculations the ten rabbits were killed at stated intervals during the succeeding six hours with the object of studying the reaction of the tissues to the "sensitized" bacilli. The pathologic study was never made, but other observations about that time tended to diminish the interest in the result.
15.
It must be confessed that we dropped this work at this point because other things seemed more important, and it was impossible to keep large animals near the laboratory with our limited facilities.
16.
Calmette and Guérin:  Ann. d. l'Inst. Pasteur , 1911, xxv, 626.
17.
Rothe, E., and Bierbaum, K.:  Veröffentl. d. Robert Koch Stiftung 1913, Nos. (8) , 9, p. 169.
18.
Ruppel and Rickman:  Ztschr. f. Immunitäasforsch. , 1910, vi, 383.
19.
Babes, V., and Proca, G.:  Ztschr. f. Hyg. u. Infectionskrankh. , 1896, xxiii, No. (3) , p. 331.
20.
Karwacki, L.:  Ztschr. f. Tuberk. , 1906, viii, 52-59.
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