Effectiveness of a COVID-19 Testing Outreach Intervention for Latinx Communities

Key Points Question Can a culturally informed community-based outreach intervention increase Latinx participation at SARS-CoV-2 testing events? Findings In this cluster randomized trial of 33 SARS-CoV-2 testing sites, the community health promoters intervention was associated with 3.84 times more Latinx individuals tested per event than control sites, and the intervention was associated with testing a greater proportion of the Latinx populace per event. Meaning The reduction of health disparities experienced by individuals identifying as Latinx during the COVID-19 pandemic may be supported by culturally informed outreach strategies.


Introduction
Data from 36 countries have indicated that population testing frequencies for SARS-CoV-2 (the virus that causes COVID-19) below 15% are associated with exponentially increasing rates of mortality. 1ta from Our World in Data of 27 countries indicate that early testing capacity defined as tests per cases is associated with lower COVID-19-related mortality rates. 2 In the first quarter of 2020, the National Institutes of Health (NIH) considered SARS-CoV-2 testing so important that the Rapid Acceleration of Diagnostics (RADx) initiative was launched to develop rapid and accurate testing and increase availability nationwide.3 As part of that mission, the RADx Underserved Populations focused on solutions to stop the spread of COVID-19 among racially and ethnically diverse populations who were disproportionately affected.3 In 2020, Latinx individuals were consistently overrepresented among cases, 4 experiencing 3 times higher COVID-19-related mortality relative to non-Latinx White counterparts.5 Currently, Latinx individuals represent 19% of the US population but still comprise 28% of COVID-19 cases nationally.6 One year into the pandemic, Latinx Oregon residents did not fare better. Mking up 13% of the state population, Latinx individuals represented 34% of COVID-19 cases and 25% of COVID-19 deaths.7 Moreover, nationally representative data indicate that Latinx individuals were underrepresented at testing sites across the US in early 2020, 8 and for every 1% increase in underrepresentation of Latinx persons based on testing site zip codes, a state's mortality rate was 1.04 percentage points more overrepresentative compared with non-Latinx individuals.
The purpose of this report is to evaluate SARS-CoV-2 testing rates from an effectiveness trial called Oregon Saludable: Juntos Podemos (Healthy Oregon: Together We Can) (OSJP).The OSJP Promotores de Salud (community health promoters, or promotores) intervention includes a culturally tailored outreach designed to increase testing among the Latinx population.Promotores models have been successful in addressing other community mental and physical health outcomes (eg, hypertension, depression, and chronic disease) [9][10][11] but have not been evaluated for SARS-CoV-2 testing.We note that ethnic and cultural labels change over time, and there is often disagreement among group members. 12Although this study's self-identified ethnicity included established NIH terms and country of origin, we use Latinx throughout instead of Hispanic or Latino, considered by some to be gender neutral and more inclusive for this diverse ethnic group. 13derrepresentation of Latinx communities in testing is influenced by factors such as lack of understanding of available resources, language barriers, and access challenges. 14,15For immigrant, migrant, and Indigenous Latinx individuals, lack of trust in institutions and misinformation also reduce the likelihood of testing. 16Reducing testing barriers and overcoming health disparities for Latinx communities require culturally responsive interventions, including not requiring health insurance, physician orders, identification, or fees and offering walk-up service. 15,17Although several programs to reduce barriers to SARS-CoV-2 testing for Latinx communities exist, 14,15,17,18 to our knowledge, this is the first randomized trial to evaluate strategies designed to accelerate SARS-CoV-2 testing among Latinx populations.On the basis of the community-based participatory intervention development and literature reviewed, we hypothesized that intervention (promotores) sites would be associated with higher numbers of Latinx individuals tested over time per site relative to county and community outreach as usual (OAU) and a higher proportion of the Latinx populace tested compared with control sites.

JAMA Network Open | Diversity, Equity, and Inclusion
Effectiveness of a COVID-19 Testing Outreach Intervention for Latinx Communities

Study Design
This cluster randomized trial used wait-listing to enroll participant sites. 19Testing participants and testing staff were blind to the intervention condition, but community-based organizations (CBOs) and county health agencies were not.Participants provided a written waiver for the use of deidentified count totals for each testing event.All consent procedures and protocols were reviewed and approved by the Committee for Protection of Human Subjects and the University of Oregon Institutional Review Board.The trial protocol is available in Supplement 1.This study followed the Consolidated Standards of Reporting Trials (CONSORT) reporting guideline extension for cluster randomized trials.
Several steps were taken for site-level randomization.First, a facilities-location-problem approach 20 was used to optimize 38 site locations in 9 Oregon counties with geomapping Latinx population concentrations to determine potential locations for testing events.Second, we focused the community engagement collaboration on instrumental aspects of testing, such as site access, optimizing visibility of events, and ensuring overall perceived safety (eg, real or perceived antagonism by community members opposed to testing, virus transmission during events, or US Immigration and Customs Enforcement authorities showing up).Given less than 10 participating counties with up to 6 possible sites, using a random-number generator, we randomized within county to minimize threats to internal validity. 19Sites were randomized to either the intervention group or the OAU wait-listed control group.

Promotores de Salud Outreach
The intervention used a community-based participatory approach that relied on partnerships with ongoing knowledge exchange among researchers, stakeholders, and the community toward development of a culturally responsive intervention. 21Promotores were bilingual (Spanish and English) and bicultural community members (N = 19) recruited through close partnerships with and hired by CBOs (eg, regional farm worker advocacy center, nonprofit organization providing integrated social services, and advocacy groups for rural underrepresented populations).A subset of the promotores consented and provided demographic information (n = 16).Of those, 7 (44%) had completed high school or General Educational Development, and 5 (31%) had some college or an associate's degree.A total of 8 (50%) had lived in the US their entire life, and 7 (44%) had lived in the US at least half of their life.
Promotores were trained to conduct outreach that highlighted common Latinx cultural values (eg, collective welfare); to disseminate information on testing events in Spanish, reasons to get tested, and COVID-19-related resources; to mitigate misinformation; and to increase trust.Strategies were tailored to local communities, including promoting testing via texting, in-person promotion at locations frequented by Latinx members (ie, specialty grocery stores, Spanish-language church services, schools, and workplaces), and advertising in print media and Latinx radio stations.All social media posts, flyers, and print outreach materials were prepared in Spanish and English.Regular meetings were held with Latinx community partners, the Oregon Health Authority, county health agencies, community and scientific advisory boards, CBOs, and promotores to (1) share up-to-date information and resources about the state's pandemic mitigation strategies, (2) plan testing event locations vis-à-vis other regional COVID-19 mitigation events, and (3) problem solve and continuously share outreach strategies twice weekly with regional CBOs.Interpreters for Mam, an Indigenous Mayan language used in Oregon, were onsite at some locations.

Outreach as Usual
Staff sample collection and testing procedures were the same across conditions.Both conditions held recurring testing events that ran for 3 to 4 hours every 2 weeks at the same time and location when possible.The OAU condition included community advertising by the study team using flyers in

Sample Collection and Testing
Anterior nares swabs were self-collected under guidance of trained study staff, placed in buffering agent, and transported to the laboratory.SARS-CoV-2 diagnostic testing was conducted at the University of Oregon's Clinical Laboratory Improvement Amendments-certified laboratory using the US Food and Drug Administration emergency use-authorized TaqPath quantitative polymerase chain reaction assay (Thermo Fisher Scientific) and analyzed using the COVID-19 Interpretive Software (Applied Biosystems) with 3 outcomes: SARS-CoV-2 positive, SARS-CoV-2 not detected, or uncertain.
Test results were returned to patients by secure email, accessed through a secure online portal, or distributed by US postal mail.Communications were available in Spanish, English, and Mam with links to posttest COVID-19 resources.

Sample
Thirty-eight testing sites were randomized (19 Promotores de Salud sites and 19 OAU sites).Data were collected from February 1 to August 31, 2021, at which time wait-listed control sites received the intervention.A total of 444 testing events were scheduled.Fifty events (11%) were canceled because of competing events held at a testing site or extreme weather conditions, including heat waves, high winds, fire danger, and unsafe air quality because of smoke, for a total analysis sample of 394 events.
Of those, the final analysis file included 212 Promotores de Salud sites and 182 OAU events.Persons 3 years or older were eligible (Figure 1). 22,23

Measures
The primary outcomes of the registered trial were number of completed populace that was tested was computed as the number of samples collected from Latinx individuals divided by the number of Latinx individuals per site census tract.

Census Tract Covariates
Five site-level census tract variables were matched to the testing site address with geocoded x-y coordinates for latitude and longitude.The census tract data 24

Weekly County SARS-CoV-2 Covariates
Two time-varying covariates potentially affecting testing rates were extracted from the Oregon Health Authority database. 25,26The SARS-CoV-2 transmission rate was measured by the total number of new weekly cases per county, and the total county population vaccination coverage was measured as the percentage of completed series of vaccinations.The weekly number of new cases was log transformed to help meet the assumption of homogeneity of variance among variables.Both the weekly cases and vaccine coverage were lagged by 1 week and then matched to the week of the testing event to meet time-ordered causal assumptions.In addition, we controlled for whether a site offered vaccines at an event.We did not offer vaccines; however, we partnered with county organizations that did so at 4% of events.

Statistical Analysis
Effectiveness hypotheses were tested with multilevel or mixed-model regressions where log sample count is regressed on the level 2 intervention effect γ 01 , site census covariates γ 02 to γ 05 , level 1 time-varying weekly cases from prior week γ 10 , and county vaccine coverage from prior week γ 20 plus residuals for predicted model (u 0 ), level 1 (u 1 ), and level 2 (r).The proportion of the Latinx populace tested was estimated as a linear mixed model using the same equation above.
Models were estimated in the R GLMMadaptive package 27 and tested with a 2-tailed α level and P < .05.

Results
The number of testing events, sample tests collected, and samples from Latinx persons are given in medium to large effect size (Cohen d = 0.74). 28Tests of time-varying random effects vs fixed effects supported specification of random effects for time-varying covariates (change χ 2 5 = 88.49,P < .001).Estimate evaluation also indicated that the model potentially underfitted zeros because of zero inflation.A post hoc sensitivity analysis for a zero-inflated negative binomial 29 adjusting for both overdispersion and zero inflation obtained identical substantive findings.Among site characteristics, higher community nativity was associated with a 38% lower likelihood of Latinx persons tested.As indicators of the pandemic climate, not surprisingly, as 1 week lagged newly confirmed cases increased over time, there were 1.75 more individuals tested per all testing events (95% CI, 1.35-2.27;P < .001).Conversely, there was a 1% decrease in the likelihood of a Latinx test for every 1% increase in county vaccine coverage, which ranged from 3% to 60%.
The intervention was associated with a 0.28 increase in the proportion of the Latinx populace being tested relative to control sites for the dependent variable scaled as the proportion of the Latinx populace ×100, or a 0.003 proportion of the raw populace count.The use of a standardized scaling of the proportion of Latinx individuals showed that the relative percentage increase was 0.53 (95% CI, 0.21-0.86) in the intervention sites relative to controls, for a medium effect size.Data also demonstrated a greater proportion of Latinx persons among all those tested (0.18; 95% CI, 0.06-0.31;P = .004).The model-based intervention effect size for primary outcomes is displayed in

Discussion
This study presents causal evidence that culturally tailored community outreach can increase SARS-CoV-2 testing rates among Latinx community members.The OSJP evaluation obtained medium to Explanatory factors accounting for health disparities and drivers of health inequity must be understood within the context of systematic historical and ongoing discrimination, chronic stress, and compromised immunologic functioning. 4As Webb Hooper and colleagues 4 argued, given documented disparities, including differential access to health insurance, health care, testing, and quality hospital care, there is an obligation to address these predictable consequences with evidence-based and culturally responsive interventions.To directly address health disparities, health scientists need to think outside the comfort zones of our clinics, laboratories, hospitals, and universities to effectively engage and meet the collective needs of our communities, just as we would prescribe correct medication to treat an individual. 30,31e OSJP project applied such a model to provide experimental evidence of these principles.
Effective participatory community engagement required building and maintaining key partner relationships across the duration of research and testing activities.Such approaches work with the Latinx community instead of developing interventions for the Latinx community and are shown to be effective for mitigating other health disparities. 32,33Engaging community partners was not without challenges.Before vaccine availability, some community partners understandably objected to  randomization and wait-listed sites.Responsivity to concerns with county officials and CBOs helped establish trust to complete the primary aim of experimentally evaluating the outreach program before releasing all sites to Promotores de Salud.Through relationship maintenance with stakeholders, we were also able to establish sustainability through state-requested contracted services for ongoing testing sites, independent of the experimental trial.All efforts were guided by and supported by a collaboration with a community and scientific advisory board that included Latinx physicians, researchers, and community leaders.

Strengths and Limitations
Strengths of the study included an experimental design with site randomization within counties to minimize threats to internal validity.Data showed no comparable differences by group condition site characteristics.Time-varying COVID-19 transmission and vaccine data were lagged to meet temporal assumptions of causal inferencing.Limitations included an overall range of significant residual variation, suggesting that unmeasured factors and factors such as implementation quality may be associated with this variation (eg, fidelity of implementation and adherence to outreach strategies).
The study covered a large portion of counties in Oregon and was generally representative of state demographic characteristics; generalizability for other underrepresented groups, however, was limited.The study was also geographically dispersed in attempts to cover rural and urban areas in a largely rural state.Beyond the scope of this study, a cost-benefit analysis would benefit the planning and resourcing of similar implementations of the Promotores model.We have established sustainability and cost sharing for ongoing testing outside the randomized study scope.

Conclusions
In this cluster randomized trial, the Promotores de Salud outreach intervention had a larger number of Latinx individuals tested per repeated testing events.Moreover, the intervention group saw an increase in the proportion of the Latinx populace tested for each testing site's census tract area.
Controlling for time-varying COVID-19 factors, the intervention's outcomes represented medium effect sizes.Findings suggest that culturally tailored outreach can be implemented or adapted to serve future needs for community engagement during a health crisis or to address ongoing health disparities.Outreach delivered by bilingual and bicultural community health promoters included (1) use of a facilities-location procedure to reduce drive time, (2) Spanish-language materials, (3) not requiring health insurance or state-issued identification, (4) not requiring physician orders, (5) not charging fees, and (6) allowing onsite walk-up registration.Other factors necessary for effective sustained community engagement included formation and maintenance of a community scientific and advisory board as partners in the implementation work.Developmental work must be iterative in partnership with community leaders, including pilot testing of all materials and protocols.
Community trust should not be expected but earned.

Figure 2 , 3 .
Figure 2, and the time-varying effect of COVID-19 transmission and vaccination coverage is shown in Figure 3. Results provide causal evidence of program effectiveness in reaching and testing a disproportionately affected underrepresented population.

Figure 2 .
Figure 2. Intervention Effect Sizes for the Primary Outcomes
were matched to site addresses using

Table 1 .
Repeated Testing Event Outcomes and Site Demographic Characteristics by Group Condition and Total Sample Results of the multilevel negative binomial and linear regressions are presented in a Values for intervention and control site types are presented as number (percentage).Positivity rates were not available by group condition or at the individual level because of Clinical Laboratory Improvement Amendments compliance and consenting requirements.Rates are estimated here in the aggregate.JAMA Network Open | Diversity, Equity, and Inclusion Effectiveness of a COVID-19 Testing Outreach Intervention for Latinx Communities JAMA Network Open.2022;5(6):e2216796.doi:10.1001/jamanetworkopen.2022.16796(Reprinted) June 16, 2022 6/12 Downloaded From: https://jamanetwork.com/ on 09/16/2023 populace tested.

Table 2 .
Incident rate ratios (IRR) greater than 1 indicate a greater likelihood of a sample test being for a Latinx individual, and IRRs of 1 or lower indicate a lower likelihood.The effectiveness hypothesis was supported.The IRR coefficient for the intervention contrast showed that intervention sites tested 3.84 (95% CI, 2.47-5.97)Latinx participants for every 1 tested at a control site, controlling for site characteristics and time-varying COVID-19 variables.The intervention effect represents a

on 09/16/2023 large
effect sizes for increasing testing rates among the Latinx population in the state of Oregon relative to wait-listed county outreach practices as usual.